不同移植路径下骨髓单个核细胞对缺血性脑梗死大鼠梗死灶血管再生的影响

目的：探讨不同移植路径下骨髓单个核细胞对大鼠缺血性脑梗死新生血管的影响。方法采用改良线栓法制造大鼠MCAO模型，根据随机数字表随机分成对照组、静脉移植组和蛛网膜下腔移植组。提取、分离和培养骨髓单个核细胞，对照组经股静脉注射注入200μl PBS液；静脉移植组经股静脉注射注入200μl含2×10^7个骨髓单个核细胞；蛛网膜下腔移植组经蛛网膜下腔注入200μl含2×10^7个骨髓单个核细胞。采用定量ELISA法对大鼠脑脊液中VEGF含量进行检测，利用免疫荧光定量观察梗死灶血管再生情况。结果骨髓单个核细胞移植后7 d、14 d和28 d，蛛网膜下腔移植组大鼠脑脊液中VEGF含量分别为（207．4±8．9）pg／ml、（171．2±10．3）pg／ml和（143．8±13．8）pg／ml，均高于静脉移植组大鼠脊液中VEGF含量，差异均具有统计学意义（P＜0．05）；移植后14 d和28 d时，蛛网膜下腔移植组BrdU阳性细胞数分别为（2043．8±514．2）个和（1834．8±307．4）个，均高于静脉移植组，均差异具有统计学意义（P＜0．05），蛛网膜下腔移植组微血管数分别为（384．6±45．1）个和（514．8±51．3）个，均高于静脉移植组，均差异具有统计学意义（P＜0．05）。结论骨髓单个核细胞移植能够增加VEGF分泌，促进脑梗死灶血管新生，经蛛网膜下腔移植效果更佳。

The effect of transplanted bone marrow mononuclear cells under different transplant paths on neovascu-larization in ischemic cerebral infarction rats

Objective To investigate the effect of treatment with transplanted bone marrow mononuclear cells under different transplant paths on neovascularization in ischemic cerebral infarction rats. Methods The MCAO models were established by intraluminal vascular suture method. All the successful models were randomly divided into control group, intravenous transplantation group and subarachnoid transplantation group according to the random number table. The Extraction,isolation and culture of bone marrow mononuclear cells were extracted,i-solated and cultured. The models in the control group were injected 200μl PBS through the femoral vein. The mod-els in the intravenous transplantation group were injected 200μl containing 2×10^7 bone marrow mononuclear cells through the femoral vein. The models in the subarachnoid transplantation group were injected 200μl containing 2× 10^7 bone marrow mononuclear cells through the subarachnoid. The VEGF levels in cerebrospinal fluid of rats were detected using quantitative ELISA method. The vascular regenerations in the infarct area were observed by using quantitative immunofluorescence. Results After transplantation of bone marrow mononuclear cells 7 d,14 d and 28 d,the VEGF levels of rat cerebrospinal fluid in subarachnoid transplantation group were （207.4±8.9）pg/ml, （171.2±10.3）pg/ml and （143.8±13.8）pg/ml,respectively,which were all higher than the VEGF levels in intra-venous transplantation group,the differences were all statistically significant （P〈0.05）. After transplantation 14 d and 28 d,the BrdU-positive cells in subarachnoid transplantation group were （ 2043. 8 ± 514. 2） and （ 1834. 8 ± 307.4） ,respectively,which were higher than the intravenous transplantation group,the differences were all statisti-cally significant （P〈0.05）. The microvessel counts in subarachnoid transplantation group were （384.6±45.1） and （514.8±51.3）,respectively,which were higher than that in the intravenous transplantation group,and the differ-ences w