社会隔离对小鼠探索行为和工作记忆的影响

目的：探讨社会隔离对小鼠的探索行为和工作记忆的影响。方法选取生后21d的昆明小鼠，随机化区组设计为对照组、隔离2周组、隔离2周群居组、隔离4周组和隔离8周组，每组10只。隔离2、4和8周组于生后21d开始，单独饲养2、4和8周，隔离2周群居组于单独饲养2周后再恢复群居至成年，同一月龄的正常饲养小鼠为对照组。采用旷场实验和自发选择性实验评估探索行为、焦虑程度和工作记忆的改变。结果在旷场实验中，隔离4周组（0．07±0．04）在中心区域所占总时间的百分比明显低于对照组（0．10±0．04），隔离8周组（0．64±0．12）在边缘区域所占总时间百分比明显高于对照组（0．43±0．08）。在T迷宫实验中，隔离2周组自发选择变换次数[第1天（5．92±0．79）次，第2天（6．67±1．3）次，第3天（7．42±1．08）次，第4天（8．17±1．27）次]明显低于对照组[第1天（6．80±1．14）次，第2天（7．60±0．84）次，第3天（8．30±0．95）次，第4天（9．20±1．32）次]。恢复群居组自发选择变换次数与相应对照组没有明显差异，隔离4周组自发选择变换次数第2天[（8．18±1．99）次]高于对照组[（6．60±2．11）次]，隔离8周组自发选择变换次数第4天[（8．29±3．04）次]高于对照组[（7．80±2．53）次]。结论隔离2周组小鼠工作记忆能力降低，可被恢复群居至成年有所改善。隔离4周组和8周组的小鼠，其探索新事物能力降低，焦虑程度增加和工作记忆能力提高。

The effects of social isolation on the exploring behavior and working memory in mice

Objective To study the effect of social isolation（ SI） on the exploratory behavior and working memory in mice. Methods The Kunming mice of postnatal 21 days were divided into the control group,SI 2 weeks group,SI 2 weeks gregarious group,SI 4 weeks group and SI 8 weeks group,according to randomized design with ten animals each. All isolated mice were isolated for 2, 4 and 8 weeks respectively, the gregarious group were housed under normal grouped housing enviroment after isolation until adult, the mice with the relative same age were control groups. All animals were measured for exploratory behavior and working memory by performing open field and T-maze after the treatment. Results In the open field,compared to the relative control group,the central area of the total time in the SI 4 weeks group（0.07±0.04） was less than the control （0.10±0.04） obviously. The central area percentage of total time in SI 8 weeks group （0.64±0.12） were more than the control （0.43±0.08）. In the T-maze,the alteration times in SI 2 weeks group （first day （5.92±0.79）,second day （6.67±1.3）,third day （7.42±1.08）,fourth day （8.17±1.27）） were less than the control （first day （6.80±1.14）; second day （7.60± 0.84）;third day （8.30±0.95）;forth day （9.20±1.32））. However,the alteration times of gregarious group showed no obvious change. Both the alteration times of SI 4 weeks （8.18±1.99） in the second day and that of SI 8 weeks （8.29±3.04） in the forth day were more than the control （6.60±2.11） and （7.80±2.53） respectively.Conclu-sions Working memory of SI 2 weeks rats decrease,which can be improved by the resocialization.SI 4 weeks and 8 weeks rats show the decreasing exploring ability and increasing anxiety and work memory.