白细胞介素-6和白细胞介素-10在急性小鼠巨细胞病毒性心肌炎中的表达

目的研究IL-6和IL-10在小鼠巨细胞病毒性(MCMV)心肌炎中的表达和在心肌保护中的作用。方法60只4周龄BALB/C小鼠随机分为2组:实验组36只小鼠,腹腔注射巨细胞病毒(MCMV)]和对照组(24只小鼠,注射3T3细胞裂解液)。应用免疫组化方法检测IL-6和IL-10在心肌组织中的表达,用病理切片观察心肌的病变。结果心肌病理结果显示,心肌组织出现灶性或弥散性炎性细胞浸润、心肌细胞的变性和坏死。心肌病理积分显示MCMV心肌炎属轻度炎症。免疫组化技术结果显示,在实验组,IL-6和IL-10蛋白主要表达在炎性浸润细胞、变性或坏死的心肌细胞,而在对照组,仅有IL-6和IL-10蛋白微量表达。结论IL-6和IL-10蛋白在MCMV心肌炎心肌组织中表达,并可能在病毒性心肌炎中起保护心肌作用。

Expression of IL-6 and IL-10 in acute myocarditis

Objective To study the expression and role of IL-6 and IL-10 in the myocardium of MCMV myocarditis.Methods Sixty BALB/C mice of 4 weeks were randomly divided into two groups:36 rats were injected intraperitoneally with MCMV and 24 rats served as control group.Immunohistochemistry technique was used to detect IL-6 and IL-10 expression in the myocardium,and myocardial lesions were observed histopathologically.Results Histopathological study on themyocardium from infected mice revealed focal or diffuse lesions characterized by inflammatory cells and degeneration or necrosis of myocytes.The myocardial lesion score showed the degree of inflammatory cell infiltration is slight in MCMV myocarditis.The positive staining signals for IL-6 and IL-10 proteins,which mainly located in the infiltrating inflammatory ceils and degenerative or necrotic myocytes,were markedly detectable,whereas there were only microamount positive findings in the myocardium of control mice.Conclusions IL-6 and IL-10 were expressed in the myocardium of viral myocarditis murine model induced by MCMV.IL-6 and IL-10 might play an important role in protecting myocardium of viral myocarditis.