| JAK/STAT1信号转导通路在博莱霉素致肺纤维化大鼠中的作用 |
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| 引用本文： | 刘金苹,翟乃亮,范贤明,高福泉,王晓芝. JAK/STAT1信号转导通路在博莱霉素致肺纤维化大鼠中的作用[J]. 山东医药, 2008, 48(34): 4-6 |
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| 作者姓名： | 刘金苹 翟乃亮 范贤明 高福泉 王晓芝 |
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| 作者单位： | 滨州医学院,山东滨州,256603;滨州医学院附属医院;泸州医学院附属医院 |
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| 摘 要： | 目的 探讨蛋白酪氨酸激酶(JAK)信号传导子和转录活化子1(STAT1)信号转导通路在肺纤维化中的作用机制.方法 将30只Wistar大鼠随机分为博莱霉素(BLM)组和生理盐水(NS)组,分别于气管内灌注BLM及NS后第7、14、28天处死.右肺支气管肺泡灌洗,进行细胞分类、计数;左肺组织匀浆测定胶原含量;免疫组化法测定STAT1、血小板源性生长因子(PDGF)和纤溶酶原激活物抑制剂1(PAI-1)蛋白的表达.结果 BLM组肺组织胶原含量在7、14、28 d均明显高于NS组(P<0.01);BLM组肺组织STAT1、PDGF蛋白表达在第7天达高峰,之后下降,第28天时仍高于NS组(P<0.05),且与支气管肺泡灌洗液中的细胞总数呈正相关(r=0.880,P均<0.01);BLM组肺组织中PAI-1表达在第7天开始升高,第28天达高峰,且与肺组织中胶原含量呈正相关(r＝0.952,P<0.01).结论 JAK/STAT1信号转导通路可能通过调节STAT1、PDGF和PAI-1表达,参与肺纤维化的形成.
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| 关 键 词： | 肺纤维化 博莱霉素 信号传导子和转录活化子1 血小板源性生长因子 纤溶酶原激活物抑制剂1 |
Research on JAK and STAT1 pathway in bleomycin-induced pulmonary fibrosis rats |
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| LIU Jin-ping,ZHAI Nai-liang,FAN Xian-ming,GAO Fu-quan,WANG Xiao-zhi. Research on JAK and STAT1 pathway in bleomycin-induced pulmonary fibrosis rats[J]. Shandong Medical Journal, 2008, 48(34): 4-6 |
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| Authors: | LIU Jin-ping ZHAI Nai-liang FAN Xian-ming GAO Fu-quan WANG Xiao-zhi |
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| Affiliation: | LIU Jin-ping1,ZHAI Nai-liang,FAN Xian-ming,GAO Fu-quan,WANG Xiao-zhi(1 Binzhou Medical College,Binzhou 256603,P.R.China) |
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| Abstract: | Objective To study the expressions of STAT1,PDGF and PAI-1 in lung tissue of rats pulmonary fibrosis model induced by bleomycin(BLM),and discuss the possible mechanism of JAK and STAT1 pathway.Methods Thirty Wistar rats were randomly divided into two groups(15 in each group): BLM group and 0.9% NaCl solution(NS) group.The BLM group was intratracheally instilled with belomycin(5 mg/kg).The NS group was intratracheally instilled with NS in the same volume.Five rats in each group were sacrificed at 7,14and 28 ... |
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