肺结核病人T淋巴细胞核仁区酸性非组蛋白表达活性的研究

目的　探讨结核病人T淋巴细胞核仁区酸性非组蛋白 (Ag-NORs)的数量，间接反映T淋巴细胞的功能，及其在结核病诊断及发病中的意义。方法 使用北京开隆公司提供的KL型免疫图像分析系统对41例正常人，77例肺结核病人和32例肿瘤病人的T淋巴细胞核仁区酸性非组蛋白表达活性进行对比，以核仁染色区面积与细胞核面积之比 (I.S%)定量反映Ag-NORs的多少。结果 正常人I.S%为 (7.20±0.95)%，肺结核病人为 (5.59±1.47)%；肿瘤病人为 (5.04±1.52)%，二者与正常人相比有显著性差异 (P＜0.001)。结核病人略高于肿瘤病人，他们之间无显著性差异。结论 结核病人酸性非组蛋白表达检测，可用于对结核病人免疫功能的检测，对估计病人的预后有临床指导意义。

The expression of nucleolar organizer regions associated of T lymphocytes in pulmonary tuberculosis

Objective To explore the role in diagnosis and pathogenesis of tuberculosis of nucleolar organizer regions (NORs) associated proteins (Ag NORs) by which studies the quantity of Ag NORs was obtained and the function of T lymphocytes was indirectly reflected.Methods 77 cases with pulmonary tuberculosis,32 cases with tumor and 41 controls individuals were analyzed with a KL Imaging System.Results I.S% of patients with tuberculosis ( n=77 ) and with tumor ( n=32 ) was significantly different with that of the normal control ( n=41 ), P< 0.001,while the difference was not found between I.S% of patients with tuberculosis and with tumor ( P> 0.05).Conclusion The expression activity assay of Ag NORs can be used to determine the immune function and evaluate prognosis of patients with pulmonary tuberculosis.