| 核糖核酸Ⅱ制备及其病毒灭活工艺的验证 |
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| 引用本文： | 朱文赫,孙妙囡,孙德军.核糖核酸Ⅱ制备及其病毒灭活工艺的验证[J].中国生化药物杂志,2008,29(5). |
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| 作者姓名： | 朱文赫 孙妙囡 孙德军 |
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| 作者单位： | 1. 吉林大学,再生医学科学研究所,生物技术药物教研室,吉林,长春,130021
2. 吉林大学,白求恩医学院,吉林,长春,130021 |
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| 摘 要： | 目的从牛胰脏制备核糖核酸Ⅱ(RNAⅡ),对巴氏灭活法灭活病毒工艺进行验证。方法以苯酚、氯仿抽提法制备,以猪细小病毒(PPV)为指示病毒,观察用巴氏灭活法(60℃,10h)灭活RNAⅡ原液中病毒的效果,用纯度、A260/A280比值、紫外特征峰、完整性、含量变化考察灭活前后制剂变化。结果制备了纯度和活性均较好的RNAⅡ,PPV滴度为7.7logTCID50/0.1mL时灭活液中未检测出PPV,经盲传3代,亦未发现特异性细胞病变,灭活前后制剂无质的变化。结论可以现行方法从牛胰脏制备较高纯度RNAⅡ,巴氏法能对RNAⅡ溶液作有效的病毒灭活处理。
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| 关 键 词： | 核糖核酸Ⅱ 制备 病毒灭活 巴氏法 |
Preparation of RNAⅡ and validation of its virus inactivation |
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| ZHU Wen-he,SUN Miao-nan,SUN De-jun.Preparation of RNAⅡ and validation of its virus inactivation[J].Chinese Journal of Biochemical Pharmaceutics,2008,29(5). |
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| Authors: | ZHU Wen-he SUN Miao-nan SUN De-jun |
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| Abstract: | Purpose To purify RNAⅡ from calf pancreas and to validate the virus inactivationl by pasteurization.MethodsRNAⅡwas purified from pancreas by extraction of phenol and chloroform and RNA solution adding the porcine parvovirus(PPV)was heated to 60℃ for 10 hours.Purity,A260/A280,special UV absorbance peak and integrity were matched before and after pasteurization.ResultsHigh purity and activity of RNAⅡ were purified,and the initial titre of PPV added into RNAⅡ solution is 7.7logTCID50/0.1 mL.The residual virus was undetectable in the virus inactivation solution and the matched itoms didn't change qualitatively.ConclusionThe method of purification of RNAⅡ and inactivation of virus is effective. |
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| Keywords: | RNAⅡ purification virus inactivation pasteurization |
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