血余炭纳米纤维膜抗菌活性的实验研究

目的:制备新型载药止血材料血余炭纳米纤维膜,并探讨其体内、体外的抗菌性能。方法:采用静电纺丝技术,将血余炭作为添加药物,以生物相容性较好的聚乳酸作为纳米纤维膜基体,制备了一种新型的载药止血材料。采用抑菌环实验法测定医用脱脂纱布、抗菌医用敷料、未载血余炭纳米纤维膜和载血余炭纳米纤维膜对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌的抑菌环直径;选用兔感染创面模型,使用材料对创面进行治疗,通过肉眼和组织病理学观察、组织内细菌计数等方法对各组材料的抗菌性能进行研究。结果:血余炭纳米纤维膜纤维直径相对较小,平均直径为400 nm,载入的血余炭与成纤维聚合物之间具有良好的相容性。血余炭纳米纤维膜在体外对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌均有一定的抑制效果;肉眼和组织学观察,血余炭纳米纤维膜治疗后的动物模型伤口炎症反应明显小于其他组;血余炭纳米纤维膜治疗后感染伤口细菌数量为(7.62±1.87)×104个.g-1组织,与空白对照组比较显著减少,差异具有统计学意义(P<0.01)。结论:血余炭纳米纤维膜具有良好的体内、体外抗菌性能。

Study on antibacterial ability of nano-flbers tunic carbonized hair loaded drug

Objective： To produce a new hemostatic agent of nano-fibers tunic carbonized hair （NTCH） loaded drug, and to test antibacterial properties in vivo and in vitro. Methods： A new type of hemostat for NTCH was prepared by using poly lactic acid as template and electrospinning technology. Antibacterial test was used to detect in vitro antibacterial properties against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa of four groups： absorbent gauze group, antiseptic medical dressing group, nano-fibers tunic and NTCH group, antibacterial zone diameters were measured and analyzed. Adopt the rabbit infection wound model, and the wound was treated by the dressings. The antibacterial properties of the agents were studied through the histological observation and the bacterial count methods in the organization. Results： The NTCH fiber average diameter of 400 nm, diameter was relatively small, with good compatibility between the loaded carbonized hair and the fiber polymer. The NTCH had antibacterial property. By the naked eye and histological observation of the NTCH for acute infected wounds of the rabbits model in the treatment group, the inflammatory reaction was less than that in the other groups. The bacterial count in the NTCH group was （7.62 ± 1.87） × 10^4. g^-1 tissue, greatly decreased compared with that of the blank group, the difference was statistically significant （P 〈 0.01）. Conclusion： The NTCH has good antibacterial properties in vivo and in vitro.