番茄红素对高糖环境下人外周血内皮祖细胞增殖、凋亡的影响

目的探讨不同浓度番茄红素对体外高糖培养环境下健康人外周血内皮祖细胞(EPCs)增殖、凋亡的影响。方法密度梯度离心法分离健康成年人外周血单个核细胞,在体外诱导分化后,用FITC标记的荆豆凝集素I(FITC-UEA-I)和Dil标记的乙酰化低密度脂蛋白(Dil-ac-LDL)双染色鉴定为内皮祖细胞。观察不同浓度番茄红素对高糖环境下的内皮祖细胞增殖、凋亡的影响。结果 33mmol/L葡萄糖明显抑制EPCs增殖,并促进其凋亡(P<0.05);番茄红素10μg/ml、30μg/ml、50μg/ml组EPCs增殖能力均明显高于0μg/ml组,凋亡率明显低于番茄红素0μg/ml组(P<0.05)。结论高浓度葡萄糖能抑制体外培养条件下人外周血EPCs的增殖、促进其凋亡;番茄红素可以保护高糖环境下EPCs的增殖能力并抑制其凋亡。

Effect of lycopene on the apoptosis of human peripheral endothelial progenitor cells cultivated in high concentration of glucose

Objective To investigate the effect of lycopene treatment on the proliferation and apoptosis of endothelial progenitor cells(EPCs) incubated in a culture medium with high concentration of glucose.Methods Mononuclear cells(MNCs) were isolated from human peripheral blood by Ficoll density gradient centrifugation.After being induced to differentiation,the endothelial progenitor cells(EPC) were identified by FITC-labeled Ulex europaeus agglutinin I and Dil labeled acetylated low density lipoprotein dual stain method.Then MTT assay and flow cytometry were used to assess the proliferation and apoptosis of EPCs.Results The glucose in a concentration of 33mmol/L significantly inhibited the proliferation and promoted the apoptosis of EPCs(P<0.05).The proliferation of EPCs in 10,30 and 50μg/ml lycopene groups were significantly higher than the 0μg/ml group.The rate of apoptosis were significantly lower than the lycopene 0μg/ml group(P<0.05).Conclusion High concentration of glucose attenuates the proliferative activity and increases the apoptotic rate of EPCs.Lycopene promotes the proliferation and reduces the apoptosis of EPCs cultivated in high glucose medium.