海参脑苷脂对H_2O_2致PC12细胞氧化损伤的保护作用

目的研究海参脑苷脂(SCC)对H2O2诱导PC12细胞氧化损伤的保护作用。方法建立H2O2致PC12细胞氧化损伤模型,MTT法检测细胞存活率;测定细胞培养液中乳酸脱氢酶(LDH)漏出量,评价细胞的损伤程度;利用荧光探针DCFH-DA对细胞内活性氧(ROS)进行荧光染色,检测荧光强度;化学比色法测定细胞内总抗氧化能力(T-AOC),黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性。结果海参脑苷脂能明显改善H2O2诱导的PC12细胞的氧化损伤,与模型组相比,SCC处理组可使细胞存活率升高,细胞培养液中LDH的漏出量明显减少(P<0.01),细胞内ROS的积累量明显降低(P<0.01),同时细胞内T-AOC和SOD活性明显增加(P<0.01)。结论 SCC对H2O2诱导的PC12细胞的氧化损伤具有一定的保护作用。

Protective effects of cerebroside from sea cucumber on the oxidative damage of PC12 cells induced by H2O2

Objective To study the protective effects of cerebroside from sea cucumber(SCC) on the oxidative damage of PC12 cells induced by H2O2.Methods An oxidative damage model of PC12 cells was induced by H2O2.The survival rate of the cells was determined by MTT method.The leakage of LDH into cell cultures was determined to evaluate the degree of cell damage.The generation of intracellular reactive oxygen species(ROS) was detected by using DCFH-DA fluorescent probe.The activity of total anti-oxidation capacity(T-AOC) was determined by chemical colorimetric method and the SOD activity was measured by xanthine oxide method.Results The injury of PC12 cells induced by H2O2 could be reduced obviously by SCC.Compared with the model group,SCC could improve the survival rate of injured cells,reduce the leakage of LDH into cell culture and reduce intracellular ROS(P<0.01),increase the intracellular T-AOC and SOD activity(P<0.01).Conclusion SCC has significantly protective effect on the injured PC12 cells induced by H2O2.The results would provide preliminary data on supporting the development of sea cucumber as a functional food,which might give benefits to our brain.