Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. |
| |
| Authors: | L Monteiro, D Bonnemaison, A Vekris, K G Petry, J Bonnet, R Vidal, J Cabrita, F M graud |
| |
| Affiliation: | L Monteiro, D Bonnemaison, A Vekris, K G Petry, J Bonnet, R Vidal, J Cabrita, and F Mégraud |
| |
| Abstract: | A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet. |
| |
| Keywords: | |
|
|
