聚合酶链反应检测SEN病毒D亚型方法的建立

目的：研究建立SEN病毒D亚型（SENV－D）聚合酶链反应（PCR）检测方法。方法：选择SENV－D开放读码框1高度保守序列，设计合成2对特异性引物，建立检测SENV－D感染的套式PCR方法。对327例无偿献血和职业献血者进行SENV感染的检测，并对部分PCR阳性产物进行克隆测序，结果：该方法特异性和灵敏度均较高，检测结果显示无偿献血人群SENV－D感染率为5．5％，职业献血人群为6．7％，两者无统计学差异，结论：我国广东部分地区无偿献血和职业献血人群中存在SENV－D亚型感染；建立的该方法适用于SENV感染的检测。

Establishment for a polymerase chain reaction of detecting SEN virus D subtype

Objective To establish a polymerase chain reaction (PCR) method for detection of SEN virus D (SENV D) DNA in serum Methods Two pair of primers were designed from ORF 1 of SENV D genome. The nested PCR method for SENV D was built and the specificity and sensitivity were tested, the 327 samples of the voluntary and commercial blood donors were detected by this method Results The specificity and Sensitivity of this PCR were ideal. The positive rate of SENV D DNA were 5 5% and 6 7% in voluntary and commercial blood donors. There was no significance difference ( P >0 05 ) between these two groups Conclusion There exist SENV D infection both in the voluntary and commercial blood donors in Guangdong province. The PCR could be used for the detection of SENV D DNA in serum