| Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA |
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| 作者姓名： | 张世忠 李良寿 欧阳修 李恩善 黄其全 汪美先 |
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| 作者单位： | Department of Epidemiology Fourth Military Medical College，Xi'an，Department of Epidemiology，Fourth Military Medical College，Xi'an，Department of Epidemiology，Fourth Military Medical College，Xi'an，Department of Microbiology，Fourth Military Medieal College，Xi'an，Department of Microbiology，Fourth Military Medieal College，Xi'an，Department of Microbiology，Fourth Military Medieal College，Xi'an |
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| 摘 要： | A sensitive and specific double-sandwich ELISA system utili-zing ascites fluid containing monoclonal antibody as a coatingantibody was developed for the detection of Clostridium botulinumtoxoid,toxin and artificially contaminated food specimens.Theminimal detectable amount(MDA)of toxoid varied from 4 to 31ng/ml.The lowest concentration for cross-reaction to botulinum AToxoid ranged from 4 to 8μg/ml.The specific index obtained wasbetween 250 and 1000.The MDA of botulin B was 16 ng/ml(4 LD50/ml),whereas that of type A toxin was larger than 32 μg/ml(301LD50/ml);the specific index was over 2000.The MDA of botulin Bin artificially contaminated food varied from 0.67 to 7.8 LD50/mland no cross-reaction was shown between types A and B.The ELISAsystem is close to RPHA in sensitivitv but is apparently superiorto the latter in specificity.
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Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA |
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| Zhang Shizhong Li Liangshou Ouyang Xue.Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA[J].Journal of Medical Colleges of PLA(China),1986(3). |
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| Authors: | Zhang Shizhong Li Liangshou Ouyang Xue |
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| Institution: | Zhang Shizhong Li Liangshou Ouyang Xue Department of Epidemiology,Fourth Military Medical College,Xi'an
Li Enshan Huang Qiquan Wang Meixian Department of Microbiology,Fourth Military Medieal College,Xi'an |
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| Abstract: | |
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| Keywords: | monoclonal antibody botulin double-sandwich ELISA |
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