多药耐药肺炎克雷伯菌可移动遗传元件研究

目的了解多药耐药肺炎克雷伯菌(MDR-KPN)耐药基因载体-可遗传元件分布状况。方法运用PCR法对20株MDR-KPN进行了接合性质粒遗传标记(traAt、rbC)、插入序列遗传标记(IS1133I、SEcp1)、转座子遗传标记(merAt、npUt、np513)、整合子遗传标记(intⅠ1i、ntⅠ2i、ntⅠ3)等4类可移动遗传元件检测。结果 trbC、ISEcp1、merAt、npUt、np513i、ntⅠ1检出阳性率分别为:55.0%、65.0%、25.0%、20.0%、50.0%、65.0%,其余4种基因均未检测到可移动性元件;20株菌株每株至少检出1种可移动遗传元件遗传标记,其中在MDR-KPN中检出ISEcp1、merA、tnpUt、rbC基因属国内首次。结论多药耐药肺炎克雷伯菌易检出各类可移动遗传元件,与医院多耐药肺炎克雷伯菌耐药机制相关的可移动遗传元件主要有trbCI、SEcp1、merAt、npUt、np513i、ntⅠ1。

Detection of Mobile Genetic Elements in Multidrug-resistant Klebsiella pneumoniae

OBJECTIVE To investigate the distribution of the drug-resistant genetic carrier-mobile genetic elements in multidrug-resistant Klebsiella pneumoniae.METHODS Four kinds of mobile genetic elements,such as conjugal plasmid genetic marks (traA and trbC),cut-in sequence genetic marks (IS1133 and ISEcp1),transposon genetic marks (merA,tnpU and tnp513) and integron genetic marks (int Ⅰ 1,int Ⅰ 2 and int Ⅰ 3) in 20 cluster MDR-KPN strains were assayed by polymerase chain reaction(PCR).RESULTS Among 20 strains of MDR-KPN,the positive rates of trbC,ISEcp1,merA,tnpU,tnp513,int Ⅰ 1 were 55.0%,65.0%,25.0%,20.0%,50.0%and 65.0%,respectively.and the other 4 genes were negative.At least one mobile genetic element was found in each MDR-KPN strain.It was first reported that ISEcpl,merA,tnpU and trbC were found positive in MDR-KPN in china.CONCLUSIONS Mobile genetic elements can be mostly found positive in MDR-KPN.trbC,ISEcp1,merA,tnpU,tnp513,and int I 1 may be the main mobile genetic elements which induce multi-resistant mechanism of MDR-KPN in our hospital.