| 纳他霉素产生菌基因组重排育种 |
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| 引用本文： | 朱惠,金志华,岑沛霖.纳他霉素产生菌基因组重排育种[J].中国抗生素杂志,2006,31(12):739-742. |
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| 作者姓名： | 朱惠 金志华 岑沛霖 |
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| 作者单位： | 1. 浙江大学宁波理工学院生物与制药工程系,宁波,315100;浙江大学材料与化学工程学院生物工程研究所,杭州,310027
2. 浙江大学材料与化学工程学院生物工程研究所,杭州,310027 |
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| 基金项目： | 国家自然科学基金;浙江省自然科学基金 |
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| 摘 要： | Streptomyces gilvosporeus SG-1原生质体经紫外线诱变并筛选链霉素抗性菌株，获得纳他霉素高产菌株。在上述高产突变株中选择4株作为亲本进行基因组重排育种，筛选得到了高产重排菌株，其中一株重排菌株S．gilvosporeus GS-74的纳他霉素产量为3574mg／L。为产量最高的亲本菌株的153％，比原始出发菌株SG-1提高1.17倍。
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| 关 键 词： | 纳他霉素 褐黄孢链霉菌 基因组重排 |
| 文章编号： | 1001-8689(2006)12-0739-04 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006年4月10日 |
Natamycin-producing strain breeding by genome shuffling |
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| Zhu Hui,Jin Zhi-hua,Cen Pei-lin.Natamycin-producing strain breeding by genome shuffling[J].Chinese Journal of Antibiotics,2006,31(12):739-742. |
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| Authors: | Zhu Hui Jin Zhi-hua Cen Pei-lin |
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| Institution: | 1. Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100; 2. Department of Chemical and Biochemical Engineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027 |
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| Abstract: | After isolating the protoplasts of Streptomyces gilvosporeus SG-1, they were mutated with UV irradiation, the streptomycin resistance mutants were screened, and the high natamycin producing strains were obtained. Among them, 4 strains with high natamycin production were selected as parent strains for genome shuffling. Consequently a high natamycin yeilding strain, S. gilvosporeus GS-74, was obtained, whose natamyein productiion reached 3574mg/L that was 153% of the parents strains and higher by 1.17 times than that of the starting strain. |
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| Keywords: | Natamyein Streptomyces gilvosporeus Genome shuffling |
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