Antiplatelet Activity of a Novel Formula Composed of Malic Acid,Succinic Acid and Citric Acid from Cornus officinalis Fruit

The present study investigated the antiplatelet activity of a novel formula composed by malic acid, succinic acid and citric acid with a ratio of 3:2:2. The IC₅₀ and inhibition of platelet aggregation induced by various agonists as well as platelet adhesion were evaluated in vitro. Of note, the IC₅₀ for the formula inhibiting adenosine diphosphate (ADP)‐induced platelet aggregation was 0.185 mg/mL. Meanwhile, the formula showed more potent inhibitory effect on platelet aggregation induced by ADP and thrombin than the single component at same concentration (0.37 mg/mL). Moreover, the formula could prevent platelet adhesion significantly without influence on platelet viability. Copyright © 2013 John Wiley & Sons, Ltd.