Bronchodilator Activity of Aerial Parts of Polygonatum verticillatum Augmented by Anti‐inflammatory Activity: Attenuation of Ca2+ Channels and Lipoxygenase |
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Authors: | Haroon Khan Muhammad Saeed Anwarul Hassan Gilani Malik Hassan Mehmood Najeeb‐ur Rehman Naveed Muhammad Muzaffar Abbas Ikram‐ul Haq |
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Institution: | 1. Department of Pharmacy, University of Peshawar, , Peshawar, 25120 Pakistan;2. Gandhara College of Pharmacy, Gandhara University, , Peshawar, Pakistan;3. Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, , Karachi, 74800 Pakistan;4. National Institute of Health, , Islamabad, 45500 Pakistan |
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Abstract: | Polygonatum verticillatum is commonly used for the treatment of asthma and inflammation. The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whilst the in vivo carrageenan‐induced paw oedema test and an in vitro lipoxygenase (LOX) inhibitory assay were used for the assessment of the anti‐inflammatory profile of PA. When tested against carbachol and K+ (80 mM)‐induced contractions, PA caused complete inhibition of isolated rabbit tracheal preparations in a dose‐dependent mode, similar to verapamil. While elucidating possible mechanism, PA shifted the Ca2+ concentration–response curves to the right, analogous to that produced by verapamil, confirming a Ca2+ channel blocker‐like activity. PA provoked profound reduction in paw oedema with a maximum protection of 60.87% at 200 mg/kg i.p. in a dose‐dependent manner which was augmented by its prominent LOX inhibitory activity (IC50: 125 µg/mL). These findings authenticated its therapeutic potential in the treatment of asthmatic and inflammatory conditions. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | Polygonatum verticillatum anti‐inflammatory bronchodilator Lipoxygenase Ca2+ channel blockers |
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