基于网络药理学探究人参-三七药对改善无症状性心肌缺血作用机制

目的:借助网络药理学研究人参-三七药对治疗无症状性心肌缺血的作用机制.方法:借助中药系统药理学数据库与分析平台(TCMSP),以"人参""三七"为检索词获取药物成分及作用靶点,运用UniProt对基因靶点标准化处理,利用Cytoscape 3.7.2软件构建成分-靶点网络图;以GeneCards平台挖掘SMI作用靶点;...

To Explore the Mechanism of Radix Ginseng-Radix Notoginseng on Improving Asymptomatic Myocardial Ischemia Based on Network Pharmacology

Objective:To study the mechanism of Radix Ginseng-Radix Notoginseng in the treatment of asymptomatic myocardial ischemia(SMI)by network pharmacology.Methods:With the help of TCMSP database,we used“Radix Ginseng”and“Radix Notoginseng”as search terms to obtain drug components and targets,and UniProt to standardize gene targets.We used Cytoscape 3.7.2 software to construct component-target network diagrams;used GeneCards platform to mine SMI targets;used Venn diagrams to obtain drug-disease intersection targets.A protein interaction network(PPI)was constructed based on the STRING database for visual analysis and screening of core targets.GO enrichment analysis and KEGG pathway analysis of core drug-disease targets were performed using Metascape database and WeChat online mapping platform.Results:A total of 22 active ingredients were obtained by analysis,4 common ingredients,125 targets,1242 SMI related targets,94 intersection targets,39 core targets.Among the main active ingredients are kaempferol,quercetin,β-guillotine,The latter two are the common components of the drug;core targets include IL6,VEGFA,TNF,TP53 and etc.GO enrichment analysis found that active regulation of cell migration,cell response to lipid,muscle cell appreciation,active oxygen metabolism process,cell response to oxidative stress and other biological processes;KGEE pathway analysis obtained AGE-RAGE signaling pathway,cancer pathway,IL-17 signaling pathway,HIF-1 signaling pathway,relaxin signaling pathway and so on in diabetic complications.Conclusion:In this study,Radix Ginseng-Radix Notoginseng drugs can play anti-inflammatory,anti-apoptosis and improve vascular permeability through regulating signaling pathway,further explained that Radix Ginseng-Radix Notoginseng treatment SMI more pathways,the mechanism is more complex.