电针对颈椎病模型大鼠椎间盘细胞外基质胶原和代谢酶活动的影响

目的:观察电针大椎穴对颈椎病模型大鼠椎间盘细胞外基质(ECM)及胞内Ⅱ型胶原(COL-Ⅱ)、V型胶原(COL-V)、基质金属蛋白酶13(MMP-13)和金属蛋白酶组织抑制因子1(TIMP-1)的影响,探讨电针治疗颈椎间盘退变的作用机制。方法:将SD大鼠随机分为假手术组、模型组、电针组和莫比可组,每组7只。建立动静力失衡性颈椎间盘退变大鼠模型。电针组取大椎穴,每次治疗30min,每天1次,14d为1个疗程,疗程之间间隔2d,共治疗2个疗程;莫比可组给予莫比可片每日0.75mg/kg灌胃治疗,共治30d。采用免疫组化法检测椎间盘中ECM、COL-Ⅱ、COL-V、MMP-13、TIMP-1的表达。结果:模型组ECM、COL-Ⅱ较假手术组明显减少(P<0.01),COL-V、MMP-13较假手术组明显增多(P<0.01,P<0.05);电针组、莫比可组ECM、COL-Ⅱ较模型组增多(P<0.01),COL-V、MMP-13较模型组减少(P<0.01,P<0.05);各组TIMP-1均无明显变化(P>0.05)。结论:电针大椎穴治疗颈椎间盘退变的机制可能与调控椎间盘细胞外基质系统有关。

Effect of Electroacupuncture Intervention on Expression of Extracellular Matrix Collagen and Metabolic Enzymes

Objective To observe the effect of electroacupuncture(EA)atDazhui(GV 14)on the contents of extracellular matrix(ECM),collagen typeⅡ(COL-Ⅱ),collagen type V(COL-V),matrix metalloproteinase(MMP)-13,tissue inhibitor of metalloproteinase(TIMP)-1in rats with cervicovertebral disc degeneration so as to explore its mechanism underlying relief of intervertebral disc degeneration.Methods A total of 28SD rats were randomly divided into sham group(n=7),model group(n=7),EA group(n=7)and medication group(n=7).The model of cervical intervertebral disc degeneration was established by trans-section of the deep neck splenius,the longest muscles of head,neck costocervicalis,head semi-spinatus muscle,supraspinous ligament and interspinal ligaments of cervical 2-7segments,etc.to produce imbalance between the dynamic and static force.EA was applied toDazhui(GV 14)for 30min,once daily for 28days,with a 2days' interval between two courses.Animals of the medication group were treated by oral administration of meloxicam tablets(0.75mg/kg)once daily for 28 days,with a 2days' interval between two courses.Immunohistochemistry was used to measure the expression of ECM,COL-Ⅱ, COL-V,MMP-13and TIMP-1in the cervicovertebral disc tissue.Results Compared with the sham group,the expression levels of ECM and COL-Ⅱproteins in the cervicovertebral disc tissue were significantly decreased in the model group(P<0.01),while COL-V and MMP-13expression levels in the model group were significantly increased(P<0.01,P<0.05).Compared with the model group,both ECM and COL-Ⅱ expression levels were considerably increased in the EA group and medication group(P<0.01),while COL-V and MMP-13expression levels were considerably down-regulated(P<0.01,P<0.05).No significant differences were found among the four groups in TIMP-1expression levels(P>0.05).Conclusion EA ofDazhui(GV 14) can effectively regulate extracellular matrix system in rats with cervical intervertebral disc degeneration,which is possibly related to its effect in relieving cervical spondylosis.