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| 温热对人胆囊癌GBC-SD细胞增殖的影响 | |
| 引用本文： | 何浪,巩春甫,曾顺泽,彭果,陈铭,戚南昌,王丹. 温热对人胆囊癌GBC-SD细胞增殖的影响[J]. 现代肿瘤医学, 2012, 20(6): 1131-1133 |
| 作者姓名： | 何浪 巩春甫 曾顺泽 彭果 陈铭 戚南昌 王丹 |
| 作者单位： | 1. 成都医学院生物医学系,四川成都,610081 2. 成都医学院临床医学,四川成都,610081 3. 成都医学院,四川成都,610081 4. 成都医学院检验医学,四川成都,610081 |
| 基金项目： | 四川省教育厅青年基金,成都医学院校基金 |
| 摘 要： | 目的:研究温热作用在体外对人胆囊癌GBC-SD细胞增殖的影响.方法:体外培养的胆囊癌GBC-SD细胞,给予43℃热作用1h,以37℃正常培养温度为对照,分别收集作用0、6、12h后的细胞.应用MTT、免疫细胞化学和Western-blot等技术,检测GBC-SD细胞经温热作用后增殖活性和PCNA蛋白表达的改变.结果:43℃热作用1h对胆囊癌细胞具有较明显的抑制效应,可有效抑制PCNA的表达.结论:43℃温热作用1h是抑制胆囊癌GBC-SD细胞增殖活性的有效热剂量. |
| 关 键 词： | 胆囊癌细胞 温热 增殖 PCNA |
Effect of hyperthermia on proliferation of human gallbladder carcinoma cell lines GBC-SD in vitro |
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| HE Lang , GONG Chunfu , ZENG Shunze , PENG Guo , CHEN Ming , QI Nanchang , WANG Dan. Effect of hyperthermia on proliferation of human gallbladder carcinoma cell lines GBC-SD in vitro[J]. Journal of Modern Oncology, 2012, 20(6): 1131-1133 | |
| Authors: | HE Lang GONG Chunfu ZENG Shunze PENG Guo CHEN Ming QI Nanchang WANG Dan |
| Affiliation: | 1Department of Biomedical Sciences,Chengdu Medical College;2Department of clinical medicine,Chengdu Medical College;3Biomedical Department of Chengdu Medical College;4Department of Examination Medical of Chengdu Medical College,Sichuan Chengdu 610081,China |
| Abstract: | Objective:To investigate the effect of hyperthermia on human gallbladder carcinoma cell lines in vitro.Methods: Human cultured gallbladder carcinoma cell GBC-SD was heated for 1 hour at 43℃.Then 0h,6h,12h later GBC-SD cells were collected respectively.The effects of hyperthermia on the proliferation of GBC-SD cells were evaluated by MTT assay.Expression of proliferating cell nuclear antigen(PCNA) was detected with immunocytochemical technique and Western-blot.Results: After lh heated at 43℃,the proliferation of gallbladder carcinoma GBC-SD cell was inhibited.Levels of PCNA were obviously down regulated in hyperthermia group compared with control group.Conclusion: One hour moderate hyperthermia treatment at 43℃ is the effective heat-dosage to inhibit human gallbladder carcinoma GBC-SD cell proliferate. |
| Keywords: | gallbladder carcinoma cell hyperthermia proliferation PCNA |
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