| nm23-H1转染对肺癌L9981细胞株整合素家族的影响 |
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| 引用本文： | 郑海霞,陆红,申东兰,彭安,何艳玲,曹菊秀.nm23-H1转染对肺癌L9981细胞株整合素家族的影响[J].现代肿瘤医学,2012,20(6):1117-1119. |
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| 作者姓名： | 郑海霞 陆红 申东兰 彭安 何艳玲 曹菊秀 |
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| 作者单位： | 1. 北京大学深圳医院,广东深圳,518036
2. 新疆医科大学第五附属医院,新疆 乌鲁木齐,830011 |
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| 摘 要： | 目的:探讨nm23-H1基因对肺癌细胞整合素分子表达的影响。方法:利用半定量RT-PCR技术检测转染前后integrinβ1、integrinβ3基因的mRNA表达;流式细胞仪检测转染前后integrinβ1、integrinβ3基因的蛋白表达。结果:转染后细胞株integrinβ1、integrinβ3 mRNA与蛋白表达下调。结论:nm23-H1基因对肺癌细胞株L9981 integrinβ1、integrinβ3 mRNA与蛋白表达具有负调控作用。
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| 关 键 词： | nm23-H1 肺癌细胞 整合素 肿瘤转移 |
Influence of nm23 -H1 tansfection on expression of integrins in lung cancer cell |
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| ZHENG Haixia
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LU Hong
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SHEN Donglan
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PENG An
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HE Yanling
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CAO Juxiu.Influence of nm23 -H1 tansfection on expression of integrins in lung cancer cell[J].Journal of Modern Oncology,2012,20(6):1117-1119. |
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| Authors: | ZHENG Haixia LU Hong SHEN Donglan PENG An HE Yanling CAO Juxiu |
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| Institution: | 1Peking University Shenzhen Hospital,Guangdong Shenzhen 518036,China;2Xinjiang Medical University the Fifth Associate Hospital,Xinjiang Wulumuqi 830011,China |
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| Abstract: | Objective:To study the infection of nm23-H1 gene on the expression of integrins in lung cancer cell.Methods: Semi-RT-PCR was used to detect the difference mRNA expression of integrinβ1 and integrinβ3 betwteen transfected and non-transfected cell lines;Flow cytometry was used to detect the difference protein expression of integrinβ1 and integrinβ3 betwteen transfected and non-transfected cell lines.Results: The expression of mRNA and protein of integrinβ1 and integrinβ3 of transfected cell line was weaker than that of non-transfected cell line.Conclusion: nm23-H1 can down-regulate expression of integrins and thus inhibit the metastasis of lung cancer cell. |
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| Keywords: | nm23-H1 lung cancer cell integrins neoplasm metastasis |
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