PDCD5对胃癌SGC-7901细胞株细胞周期的影响

目的:通过检测PDCD5蛋白对胃癌细胞株SGC-7901的细胞周期的影响,探讨PDCD5蛋白对胃癌的作用。方法:培养胃癌细胞株SGC-7901。构建pEGFP-C1-PDCD5质粒并转染SGC-7901细胞,使其表达PDCD5蛋白。另外在SGC-7901细胞的培养基中,加入重组人PDCD5(rhPDCD5)蛋白直接作用。采用MTT比色法检测细胞增殖情况,流式细胞术检测细胞周期,免疫荧光技术和Western blot检测相关周期蛋白的变化。结果:在以上两种作用方式下,SGC-7901的增殖均受到抑制,细胞增殖率明显降低(P<0.01),细胞出现G0/G1期阻滞(P<0.05)。经过两种作用方式处理后的细胞周期蛋白Cyclin D1和Cyclin E明显下降(P<0.01)。结论:转染PDCD5质粒与rhPDCD5蛋白均能够起到对胃癌细胞株SGC-7901周期阻滞的作用。且两种方式作用的效果和机制无明显差异。可以为临床胃癌治疗提供新的思路。

The effect of PDCD5 on cell cycle of gastric cancer cell

Objective:To measure the effect of PDCD5 on cell cycle of SGC-7901 and evaluate its role in gastric cancer.Methods: The gastric cancer cell SGC-7901 was cultured.We constructed the plasmid pEGFP-C1-PDCD5 and transfected into SGC-7901.The recombinant human PDCD5 protein was added into the culture solution of SGC-7901.MTT was used to detect the proliferation rate of the cells.Cell cycle was detected by FCM.The changes of related cell cycle proteins were analyzed by Western blot and immunofluorescence technique.Results: Under the two mode of action,the proliferation rate of SGC-7901 in treated cells was significantly lower than untreated ones(P<0.05).The G0/G1phase increasd in all treated cells(P<0.05).And G0/G1phase related proteins Cyclin D1 and Cyclin E were decreased in treated cells(P<0.01).Conclusion: Both the two mode of action can lead gastric cancer cells to G0/G1phase arrest.There were no differences between the two mode of action in molecular mechanisms and effect.The results may provide a new method to treat gastric cancer.