电针治疗部分损伤帕金森病大鼠的实验研究

在我国 ,应用针刺疗法治疗帕金森病 (ＰＤ)已经有几十年的历史。临床实践证明 ,它可以在一定程度上缓解ＰＤ患者的病情 ,提高生存质量。并且针刺对其他一些神经系统疾病 ,如视神经萎缩、脊髓损伤、小儿脑发育不良等也有一定的治疗作用 ,但针刺治疗产生效果的机制至今不清。本研究中 ,我们用不同频率的电针刺激线刀损毁内侧前脑束 (ＭＦＢ)制备ＰＤ大鼠模型 ,并探讨电针能否减轻大鼠的异常旋转行为 ,阻止ＤＡ能神经元的退行性改变并增强ＤＡ能神经元内ＧＤＮＦ和ＢＤＮＦ基因的表达。主要的结果如下 :①用ＫＯＰＦ线刀单侧损毁大鼠ＭＦＢ 2周…

A Mechanism Study on the Effect of Electroacupuncture on A Partially Lesioned Rat Model of Parkinson's Disease

Acupuncture has been applied in the treatment of Parkinson's Disease (PD) in China for over decades. Clinical practice revealed that it could relieve the suf ferings of PD patients and improve their life quality. Besides, acupuncture is a lso effective for other neurological disorders such as optic nerve atrophy, spin al cord injury and brain dysplasia. But the mechanism underlying the phenomenon is still unknow n. In the present study, we gave high and low frequency electroacupuncture (EA) stimulation to the medial forebrain bundle (MFB) transected rats in order to fi nd whether EA could attenuate the abnormal rotational behavior and dopaminergic neuron degeneration or increase the gene expression of GDNF and BDNF in the dopa minergic neurons. The main findings are: ① Transection of the MFB with a wire k nife resulted in abnormal ipsilateral rotational behavior of rats induced by amp hetamine and reduction of DA in the striatum to about 50% two weeks after the tr ansection. ② Both chronic low (2 Hz) and high (100 Hz) frequency EA stimulation were effective in attenuating the rotational behavior within 2 weeks of MFB tra nsection. The same effect could last for at least 4 weeks in the animals stimula ted with high frequency EA but not low frequency EA. ③ The content of DA in the striatum was increased significantly in the unlesioned side of both the low and high frequency EA stimulated rats. No increase had been found in the lesioned s ide. ④ High frequency EA could increase the gene expression of GDNF on the unle sioned side of substantia nigra reticular (SNr) region, and both sides of the gl obus pallidus, while low frequency EA was effective in increasing gene expressio n of GDNF in the unlesioned side of globus pallidus. ⑤ High frequency EA could attenuate the degeneration of dopaminergic neurons in the SNc. The low frequency EA had no effect. ⑥ High frequency EA, but not the low frequency, could increa se the gene expression of BDNF in the SNc and the ventral tegmental area (VTA) o f the lesioned side of rats after MFB transection, indicating that high frequenc y EA stimulation could be more effective than the low frequency EA in accelerati ng the synthesis of endogenous neurotrophic factors and in attenuating the degen eration of dopaminergic neurons.