肺癌组织中错配修复基因hMLH1、hMSH2表达的研究

目的:探讨人类错配修复基因hM LH 1、hM SH 2在肺癌组织中的表达及意义。方法:运用免疫组织化学S-P法对56例肺癌组织中hM LH 1、hM SH 2的表达进行检测。结果:56例肺癌组织中hM LH 1的阳性表达率为35%,hM SH 2阳性表达率为28.6%,分化程度高者阳性率显著高于分化程度低者(P<0.01),有淋巴结转移者hM LH 1及hM SH 2阳性率低于无淋巴结转移者(P<0.05),不同病理组织学类型之间hM LH 1及hM SH 2表达无显著差别(P>0.05)。结论:hM LH 1及hM SH 2基因的缺陷与肺癌的发生发展过程并与其分化程度及有否淋巴结转移有关。

Study of Human Mismatch RepairGene(hMLH1/hMSH2) in Lung Cancer

Objective: The aim of this study was to analyze expression of hMLH1 and hMSH2 mismatch repair proteins in terms of the clinicopathological parameters in lung cancer.Metheds: hMLH1,hMSH2 expression in tumor tissue sections was determined using S-P immunohistochemical staining.Result: Of the 56 cases,immunohistochemical analysis showed that the positive expression rate of hMLH1 and hMSH2 was 35% and 28.6% respectively.The positive rate of hMLH1/hMSH2 expression in the patients with high and middle differention grades was significantly higher than that of the others(P<0.01),meanwhile,the positive rate of cases with lymph node metastasis was lower than that of the patients without lymph node metastasis(P<0.05).It wasn't positively correlated with pathological types.Conclusion: The abnormal expression of hMLH1/hMSH2 might be involved in the carcinogens process of lung cancer and correlated with differention grades and lymph node metastasis.