转sck基因水稻中CpTI蛋白的细胞内定位

目的转sck基因水稻中CpTI蛋白细胞内定位。方法运用免疫组织化学方法,应用免疫电镜技术,观察在转sck基因水稻根部细胞、叶片细胞中CpTI蛋白的定位情况。结果在转sck基因水稻根尖部细胞的内质网中和内质网附近有胶体金颗粒大量存在,细胞的质体、叶绿体、细胞质、细胞核中也散在一些胶体金颗粒,在对照水稻的根部内质网和其他部位未发现胶体金颗粒存在。水稻叶片细胞由于有叶泡的存在,内质网不明显,但是细胞核、细胞质和质体内也发现胶体金颗粒,在非转基因水稻的叶片中未发现胶体金颗粒。结论转sck基因水稻中的CpTI蛋白在内质网中大量存在,在细胞的质体、叶绿体、细胞质、细胞核中也存在少量CpTI蛋白。

Study on endoplasmic reticulum localization of CpTI protein in sck transgenic rice

OBJECTIVE: To study the endoplasmic reticulum (ER) localization of CpTI protein in sck transgenic rice. METHODS: Immunohistochemistry and immune electron microscopy (IEM) were used to observe the localization of CpTI protein of root and leaf cell in sck transgenic rice. RESULTS: The endoplasmic reticulum localization of CpTI protein were observed by IEM. Colloidal gold granules were found in ER of root cell of sck transgenic rice and in root cells of the control plant colloidal gold granules were not observed. In plastid, chloroplast, cytoplasm, karyon of root cell of sck transgenic rice, a little of colloidal gold granules were also observed. The ER in the leaf cell was not observed because of the presence of vacuole, but some colloidal gold granules in cytoplasm, karyon and plastid of leaf cells were observed in sck transgenic rice. Colloidal gold granules were not observed in leaf cells of the control plant. CONCLUSION: It seemed that CpTI protein was mainly localized in the ER of the cell from sck transgenic rice and in other organelles there was also a little CpTI protein.