| 水蛭提取物对血管内皮细胞VEGF受体-2表达的影响 |
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| 引用本文： | 熊欣,彭琦,郑燕林. 水蛭提取物对血管内皮细胞VEGF受体-2表达的影响[J]. 国际眼科杂志, 2009, 9(1): 41-43. DOI: 10.3969/j.issn.1672-5123.2009.01.013 |
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| 作者姓名： | 熊欣 彭琦 郑燕林 |
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| 作者单位： | 成都中医药大学,中国四川省成都市,610072;成都中医药大学附属医院眼科,中国四川省成都市,610072 |
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| 摘 要： | 目的:研究水蛭提取物对凝血酶诱导的RF-6A细胞(恒河猴视网膜脉络膜血管内皮细胞)跨膜受体VEGFR2表达的影响,从而探讨中药水蛭用于防治新生血管形成中的作用。方法:将培养细胞分成水蛭组,凝血酶组,凝血酶+水蛭组及空白组。在前期实验基础上选择64g/L水蛭提取液及20NIHU/mL凝血酶。8h后,采用免疫组织化的方法检测各组细胞VEGFR2的表达,并进行图像分析。结果:免疫组化分析:与空白组比较水蛭组RF-6A细胞膜上VEGFR2的表达明显降低,降低程度高于凝血酶组,小于混合组,Sig=0.000,P<0.01各组间的差异有统计学意义。结论:水蛭提取物可使内皮细胞跨膜受体VEGFR2表达明显减少,推测其对VEGF受体介导的跨膜信号传导途径的作用可能是通过竞争性地抑制VEGF与其受体的结合。
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| 关 键 词： | 水蛭提取物 RF-6A细胞 凝血酶 VEGFR2 免疫组化 |
Effect of the leech extracts from percolation on the expression of transmembrane receptor VEGFR2 of RF-6A cells |
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| Xin Xiong,Qi Peng,Yan-Lin Zheng. Effect of the leech extracts from percolation on the expression of transmembrane receptor VEGFR2 of RF-6A cells[J]. International Eye Science, 2009, 9(1): 41-43. DOI: 10.3969/j.issn.1672-5123.2009.01.013 |
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| Authors: | Xin Xiong Qi Peng Yan-Lin Zheng |
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| Affiliation: | Xin Xiong1,Qi Peng1,Yan-Lin Zheng21Chengdu University of Traditional Chinese Medicine,Chengdu 610072,Sichuan Province,China,2Department of Ophthalmology,Affiliated Hospital of Chengdu University of TCM |
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| Abstract: | AIM:To study the effect of the leech extracts from percolation on the expression of transmembrane receptor VEGFR2 of RF-6A cells(rhesus monkey retinal vascular endothelial cells)in vitro,and to infer its role on preventing the newborn blood vessel.METHODS:The culture cells were divided into four groups:leech group,thrombin group,thrombin + leech group,and blank control group.Based on the previous experiments,64g/L leech extracts and 20NIHU/mL thrombin were chosen.After 8 hours,the expression of VEGFR2 on ea... |
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| Keywords: | VEGFR2 |
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