碱性成纤维细胞生长因子对预构皮瓣血管化及成活的影响

目的：探讨碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）对预构皮拉血管化的作用及成活的影响。方法：将新西兰大白兔耳中央动、静脉通过皮下隧道植入颈部皮瓣下方，实验组滴入9цg bFGF，对照组滴入0.2ml生理盐水。于术后1，2，3周观察血管芽与皮瓣血管沟通及皮瓣成活范围。结果：预构皮瓣术后1，2周，实验组血管芽及皮瓣成活率明显高于对照组。结论：局部应用bFGF对预构皮瓣的血管芽发生有促进作用，有利于预构皮瓣的成活。

The effect of basic fibroblast growth factor( bFGF) on neovascularization and prefabricated flap survival

OBJECTIVE: To observe the effect of basic fibroblast growth factor (bFGF) on neovascularization and prefabricated flap survival. METHODS: Male New-Zealand rabbits weighting 2.0-2.5 kg were used in this study. The experimental model used a prefabricated neck flap, supplied by the transferred and implanted central vascular bundle of the ear. 9 micrograms bFGF and 0.2 ml of normal saline was instilled in the vascular pedicle of the experimental and the control group respectively. After 1, 2, 3 weeks of operation, the neovascularization was studied by confocal laser scanning microscope (CLSM) and flap survival was observed. RESULTS: The neovascularization and survival of the prefabricated flap was different in the experimental and the control groups. The experimental group was better than the control group. CONCLUSION: bFGF treatment improved sprouting of the implanted vessel and the prefabricated flap survival.