血小板添加液保存单采血小板的研究

目的研发一种新型血小板添加液(H-sol),并评价其对血小板的保存效果。方法新型血小板添加液(H-sol)的组成成分:氯化钠80.0mmol/L、醋酸钠25.0mmol/L、氯化钾5.0mmol/L、氯化镁2.0mmol/L、枸橼酸钠15.0mmol/L、葡萄糖15.0mmol/L、碳酸氢钠13.0mmol/L、磷酸二氢钠4.0mmol/L、L-精氨酸180.0μmol/L。将超浓缩单采血小板(PLT≥10×109/ml)悬浮在H-sol和100%血浆(对照组)介质中,置22℃±2℃振荡条件下保存,分别于1、5、7d取样检测血小板计数(PLT)、血小板平均体积(MPV)、血小板体积分布宽度(PDW)、pH、葡萄糖、乳酸、血小板低渗休克反应(HSR)、血小板形变能力(ESC)、CD62P表达率。结果血小板保存至7d时,H-sol组(含<10%的血浆)与对照组比较,PLT、MPV、PDW、CD62P表达率、HSR、ESC的差异无统计学意义(P>0.05),其pH高于对照组(P<0.01),1～7d葡萄糖平均消耗量和乳酸平均产生量明显低于对照组(P<0.01)。结论单采血小板在H-sol中的保存效果与100%血浆相同,血小板在H-sol中保存能更好地维持pH的稳定。

In Vitro Quality of Apheresis Piatelets Stored in a New Additive Solution (J-sol)

Objective To develop a new platelet additive solution(H-sol) and evaluate its effectivity.Methods The ingredientsofthenewplateletadditivesolution(H-sol)wereasfollows:sodiumchloride80.0mmol/L,sodiumacetate 25.0 mmol/L,potassium chloride 5.0 mmol/L,magnesium chloride 2.0 mmol/L,sodium citrate 15.0 mmol/L,glucose 15.0 mmol/L,sodium bicarbonate 13.0 mmol/L,sodium dihydrogen phosphate 4.0 mmol/L,L-arginine 180.0 μmol/L.The hyperconcentrated apheresis platelets(PLT≥10×109/ml)were respectively suspended in H-sol and 100% plasma(control) and preserved with continuous shaking at 22℃±2℃.Samples were spiked at 1st day,5th day and 7th day to investigate their platelet count(PLT), mean platelet volume(MPV),platelet distribution width(PDW),pH,concentration of glucose and lactate,hypotonic shock response(HSR),extent of shape change(ESC) and expression of CD62P.Results PLT,MPV,PDW,expression of CD62P,HSR and ESC showed no statistical differentiae between platelets preserved in H-sol(containing <10% plasma) and 100% plasma until the 7th day(P>0.05).pH was higher in H-sol than in control(P<0.01).The average consumption of glucose and production of lactate in H-sol were obviously lower than that in control during 1st day to 7th day(P<0.01).Conclusions Apheresis platelets preserved in H-sol showed the same effects with 100% plasma and had a more stable pH.