荧光原位杂交技术在泌尿生殖肿瘤中的应用

随着分子细胞遗传学的快速发展,荧光原位杂交（fluorescence in situ hybridization,FISH）技术已广泛应用于临床和科学研究。FISH技术综合了荧光信号的高度灵敏性、无放射性、直观性及原位杂交的准确性,通过检测样本中基因或染色体的异常改变而应用于遗传性疾病、肿瘤的研究及临床诊断和治疗监测。本文就荧光原位杂交技术在常见的泌尿生殖肿瘤中的应用进行综述。

The applications of fluorescence in situ hybridization technique in tumors of the genitourinary

With the rapid development of molecular cytogenetics,fluorescence in situ hybridization（FISH） technique has been widely used in clinical and research.FISH has the high sensitivity of fluorescence signal,non-radioactive,intuitive,and the accuracy of in situ hybridization,can be used in detecting the abnormal gene or chromosome change of genetic diseases,cancer research and clinical diagnosis and treatment monitoring.In this paper,the applications of fluorescence in situ hybridization technique in diagnosis and treatment of common urinary tract cancer are reviewed.