大鼠脑血管内皮细胞中神经肽Y的Y1受体

目的 对脑血管内皮细胞中神经肽Ｙ（ＮＰＹ）的Ｙ１受体进行精确定位。方法 用针对ＮＰＹ的Ｙ１受体Ｃ末端肽片段的一种纯化的抗血清，用免疫组织化学方法和包埋前免疫金－银加强法观察Ｙ１受体在脑血管内皮细胞中的分布。结果 大脑皮质和髓质、丘脑、下岳脑、小脑和脑干的微血管有散在的含Ｙ１受体免疫反应的（－Ｉｒ）内皮细胞，孤立的脑底动脉及其分支的一些内皮细胞也具有Ｙ１受体免疫反应性（－ＬＩ），Ｙ１受体－ＬＩ分布于

Y1 receptor of neuropeptide Y in endothelium of cerebral blood vessels in rats

AIM To accurately localize Y_1 receptor of neuropeptide Y(NPY) in the endothelium of cerebral blood vessels. METHODS An affinity-purified antiserum against a C-terminal peptide fragment of Y_1-R was used with the immunobiochemistry and pre-embedding electron gold-silver immunostaining. RESULTS Disperse Y_1-R-Ir endothelium wasobserved in the micro-blood vessels of the brain cortex and brain medulla, campus, hippocampus, cerebellum and brainstem, and also in the isolated basal cerebral arteries and their branchs. There was Y_1-R-LI in the plasmalemma and plasma facing the smooth muscle cells and the lumen of the blood vessels. CONCLUSION NPY from the blood, endothelium and the nerve around the blood vessels may play a role in the regulation of cerebral blood flow and stimulate the proliferation of the smooth muscle cells via Y_1-R.