| HPLC法测定野菊花中绿原酸的含量 |
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| 引用本文： | 姜丽霞,徐世芳. HPLC法测定野菊花中绿原酸的含量[J]. 中国药品标准, 2003, 4(6): 54-56 |
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| 作者姓名： | 姜丽霞 徐世芳 |
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| 作者单位： | 浙江省医学科学院药物研究所,杭州,310013 |
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| 摘 要： | 目的;建立野菊花中绿原酸的含量测定方法并测定其含量。方法:用 50％甲醇回流提取;HPLC法测定,分析色谱柱为Inertsil C_(18)(5μm,4.6mm×150mm),流动相为乙腈-0.4％磷酸水溶液(12:88),UV346nm检测。结果:精密度试验批内(n=9)RSD为1.3％,批间(n=9)RSD为1.8％;平均回收率(n=5)为100.3％,RSD为2.0％。分析了3种市售样品,野菊花中绿原酸含量为0.33％～0.36％。结论:方法简便,可靠;绿原酸可作为野菊花的质量控制指标。
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| 关 键 词： | 野菊花 绿原酸 中药材 含量测定 高效液相色谱法 |
HPLC Determination of Chlorogeric Acid in Flos Chrysanthemiindici |
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| Jiang Lixia and Xu Shifang. HPLC Determination of Chlorogeric Acid in Flos Chrysanthemiindici[J]. , 2003, 4(6): 54-56 |
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| Authors: | Jiang Lixia and Xu Shifang |
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| Affiliation: | Instetute of Medico.Materia.Zhejiang Academy of Medicine, Hang zhou Zhejiang 310013;Instetute of Medico.Materia.Zhejiang Academy of Medicine, Hang zhou Zhejiang 310013 |
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| Abstract: | Object: To establish a method for the determination of chlorogeric acid in Flos Chrysanthemi Indici. Methods powdered herb was extracted by reflux 2h with 50% methanol. The chromatographic system consisted of Inertsil c18 column and mobile phase of MeCN -0. 4% H3PO4(12 : 88), and the detective wavelength was at 346nm. Results:The method was precise with RSD of 1. 3% for within-day variation (n = 9) and of 1. 8% for between-day variation (n = 9). Average recovery for chlorogeric acid was 100. 3% with RSD 2. 0%(n = 5). 3 samples were analyzed and the contents of chlorogeric acid were from 0. 33% to 0. 36%. Conclusion:Chlorogeric acid can be applied as the quality index for Flos Chrysanthemi Indici. |
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| Keywords: | Chlorogeric acid Flos Chrysanthemi Indici HPLC |
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