乙型肝炎病毒基因型和亚型与病毒感染相关性研究 |
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引用本文: | 陈俊梅,李卓,郝娃,牛京勤,严艳. 乙型肝炎病毒基因型和亚型与病毒感染相关性研究[J]. 中国误诊学杂志, 2010, 10(32): 7817-7819 |
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作者姓名: | 陈俊梅 李卓 郝娃 牛京勤 严艳 |
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作者单位: | 首都医科大学附属北京佑安医院,北京100069 |
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摘 要: | 目的探讨乙型肝炎病毒(HBV)基因型和亚型与慢性乙型肝炎(CHB)发病的关系。方法对454例CHB患者,定量PCR方法检测HBV DNA为阳性,再以型特异性引物PCR法检测HBV基因型及其亚型。结果 (1)454例HBV DNA阳性的CHB患者其中HBV B基因型68例(14.9%);其中Ba亚型35例,Bj亚型3例,未分B亚型30例,C基因型338例(74.5%),C基因型中C1亚型3例,C2亚型285例,未分型50例。B+C混合基因型48例(10.6%),均为Ba+C2基因型;(2)B与C基因型比较,B基因型中HBeAg阴性的患者多于HBeAg阳性的患者(χ^2=17.69,P〈0.001)。CHB患者中C基因型的HBeAg阳性率明显高于B基因型(χ^2=17.025,P=0.000)。(3)定量PCR检测B基因型HBV水平低于其他基因型;C基因型HBV复制高于其他基因型。结论我国CHB患者中HBV基因型以C基因型为主;HBV基因亚型中Ba与C2亚型是主要的亚型,不同的HBV亚型间病毒载量差异有统计学意义;HBeAg阳性或HBeAg阴性的CHB患者中HBV基因型分布不同;HBV基因型和亚型的临床意义值得动态观察。
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关 键 词: | 肝炎病毒,乙型/遗传学 肝炎,乙型,慢性/遗传学 |
Clinical and Virological Manifestation of Hepatitis B Virus (HBV) Genotypes and Subtypes |
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Affiliation: | CHEN Jun mei,LI Zhuo,HAO Wa,et al.(Beijing You'an Hospital Affiliated to Caipital Medical University,Beijing 100069,China ) |
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Abstract: | Objective To detect the genotypes and subtypes of hepatitis B virus(HBV) in patients with chronic hepatitis B(CHB).Methods Using the type and subtype-specific primers to detect 454 patients with CHB in Beijing.Results(1)The genotype B、 C and mixed infection of B/C were 68(14.98%),338(74.45%) and 48(10.57%) separately;The subtypes Ba,Bj were 35(51.47%) and 3(4.41%) cases,with 30(44.12%) neither Ba or Bj in B genotypes.In genotype C,only 3 cases(0.89%) were subtype C1,285 cases(84.32%) were subtype C2,with the remaining 50(14.79%) were neither C1 nor C2;All the 48 mixed B/C genotype were subtype Ba+C2;(2)There were significantly different between the genotype B and C among patients with positive and negative HBeAg.(3)the level of HBV DNA in genotype B were lower than other genotype;the level of HBV DNA in genotype C were higher than B genotype.Conclusion Our results suggest:(1)The genotype C is the majoy type in patients with CHB in Beijing;(2)The Ba and C2 are most important subtypes in the patients.The level of HBV DNA are significantly among different subtypes;(3)the frequencies of genotype distributions are different between HBeAg positive and negative groups. |
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Keywords: | Hepatitis B virus/genetics Hepatitis B Chronic/genetics |
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