| HPLC法测定虫草被孢菌胶囊中腺苷的含量 |
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| 引用本文： | 吴文,刘晓鸿,朱满芳.HPLC法测定虫草被孢菌胶囊中腺苷的含量[J].中国药物警戒,2013,10(9):540-542. |
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| 作者姓名： | 吴文 刘晓鸿 朱满芳 |
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| 作者单位： | 1. 安徽医科大学第三附属医院,合肥市第一人民医院药学部,安徽合肥230061
2. 安徽圣鹰药业有限公司,安徽合肥,230031 |
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| 基金项目： | 安徽省“十二五”临床重点专科基金资助,卫科秘[2013]228号. |
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| 摘 要： | 目的 建立虫草被孢菌胶囊中腺苷含量测定方法.方法 采用高效液相色谱法测定虫草被胞菌胶囊中腺苷的含量.采用Hypersil C18色谱柱(4.6 mm×250 mm,5μm)；流动相:磷酸二氢钾溶液(0.05 mol·L-1)-甲醇(85∶15)为流动相,流速1.0 mL· min-1,检测波长为260hm.结果 腺苷在1.011～ 50.55μg·mL-1范围内线性关系良好,线性方程为:A=60901C-2347.7,r=1；平均回收率为100.49％(n=6,RSD=1.1％).结论 本法操作简便,不受杂质干扰,精密度好,结果准确可靠,可用于虫草被孢菌胶囊的质量控制.
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| 关 键 词： | HPLC 含量测定 虫草被孢菌胶囊 腺苷 |
Using HPLC to Determine Adenosine in Cordyceps Mortierella Mycelia Capsules |
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| WU Wen
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LIU Xiao-hong
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ZHU Man-fang.Using HPLC to Determine Adenosine in Cordyceps Mortierella Mycelia Capsules[J].Chinese JOurnal of Pharmacovigilance,2013,10(9):540-542. |
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| Authors: | WU Wen LIU Xiao-hong ZHU Man-fang |
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| Institution: | WU WenI LIU Xiao-hongI ZHU Man-fang; (1Department of Pharmacy, The Third Hospital Affiliated to Anhui Medical University, Hefei First People＇s Hospital, Anhui Hefei 230061, China; 2Shengying Pharmacy Co.Ltd. Anhui Hefei 230031, China) |
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| Abstract: | Objective To establish a method of determining adenosine in Cordyceps Mortierella Mycelia Capsules. Methods We used HPLC to determine adenosine in Cordyceps Mortierella Mycelia Capsules. In this method, Hypersil C18 column（4.6 mm× 250 mm,5μm） was used with the mobile phase of KDP buffer solution（0.05 mol·L-）-methanol（85：15）. The flow rate was 1.0 mL＇min-1, and the wavelength was 260 nm. Results The linear relationship of adenosine within the range of 1.011-50.55μg·mL-1 is fine. The linear equation is A=60901C-2347.7, r=l, and the average recovery rate is 100.49%（n=6, RSD=l.1%）. Conclusion Using HPLC to determine adenosine in Cordyceps Mortierella Mycelia Capsules is not only easy to operate but also without the interference of impurity. It has good precision and the result is reliable. It can be used as a method in the quality control of Cordyceps MortiereUa Mycelia Capsules. |
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| Keywords: | HPLC determination Cordyceps Mortierella Mycelia Capsules adenosine |
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