| 比色法测定菌核侧耳中三萜类成分的含量 |
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| 引用本文： | 余志坚,包水明,李荣同. 比色法测定菌核侧耳中三萜类成分的含量[J]. 时珍国医国药, 2006, 17(6): 911-911 |
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| 作者姓名： | 余志坚 包水明 李荣同 |
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| 作者单位： | 东华理工学院,江西,抚州,344000 |
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| 基金项目： | 东华理工学院硕士启动基金资助项目（No.DHS-0428） |
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| 摘 要： | 目的对菌核侧耳中的三萜类成分进行含量测定。方法采用比色法,测定了菌核侧耳菌丝体和子实体三萜类化合物的含量,还优选了其显色条件,5%香草醛-冰醋酸最佳用量为0.2 m l,高氯酸最佳用量为1 m l。结果菌核侧耳子实体总三萜含量为1.16%,菌丝体为1.31%,RSD为1.05%。结论该方法简便、迅速可靠。
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| 关 键 词： | 菌核侧耳 比色法 三萜类 |
| 文章编号： | 1008-0805（2006）06-0911-01 |
| 收稿时间： | 2005-07-25 |
| 修稿时间： | 2006-01-21 |
The Content of Triterpenoids in Pleurotus tuber-regium (Fr.) Sing Determined by Colorimetry |
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| YU Zhi-jian,BAO Shui-ming,LI Rong-tong. The Content of Triterpenoids in Pleurotus tuber-regium (Fr.) Sing Determined by Colorimetry[J]. Lishizhen Medicine and Materia Medica Research, 2006, 17(6): 911-911 |
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| Authors: | YU Zhi-jian BAO Shui-ming LI Rong-tong |
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| Affiliation: | Department of Biology, East China College of Science and Technology, Fuzhou, Jiangxi 344000, China |
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| Abstract: | ObjectiveTo determine the content of triterpenoids from mycelial and fruit body of Pleurotus tuber-regium(Fr.) Sing were determined by colorimetry.MethodsThe optimum volume of 5% vanillin-glacial acetic acid was 0.2 ml,and perchloric acid was 1 ml.ResultsThe results shaved triterpenoids composition of fruit body is 1.16%,the mycelium was 1.31%,RSD was 1.05%.ConclusionThis method is simple and convenient,fast and reliable.The Color Conditions were optimized. |
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| Keywords: | Pleurotus tuber-regium(Fr.) Sing Colorimetry Triterpenoids |
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