三氧化二砷抑制体外培养小鼠气道成纤维细胞增殖及c-myc与c-sis表达

目的：探讨三氧化二砷 (As2O3))对体外培养的小鼠气道成纤维细胞 (FB)增殖及原癌基因c-myc与c-sis表达的影响。 方法： 按不同药物分7组，在体外培养的FB中分别加入浓度为0.25、0.5、1.0、2.0和4.0 μmol/L的As2O3，在1、3、5和7 d后用记数法观察FB生长情况，并用流式细胞仪（FCM）检测不同浓度药物 对各组FB增殖的影响及各组c-myc与c-sis的阳性表达率。 结果： 各种实验浓度的As2O3对FB均有抑制作用，并有时间剂量效应。流式细胞仪分析显示，随着浓度的增高，G1期细胞比例逐渐增高，G2/M期细胞比例降低，浓度为2.0和4.0 μmol/L的As2O3能显著抑制c-myc与c-sis的表达。 结论： As2O3能抑制FB的增生，其机理可能与其下调c-myc与c-sis的表达有关。

Inhibitory effect of arsenic trioxide on the proliferation of airway fibroblast and the expression of c-myc and c-sis in vitro

AIM: To study the effects of arsenic trioxide (As2O3) on mouse airway fibroblast (FB) proliferation and expression of c-myc and c-sis in cultured FB. METHODS: The cultured FB was divided into seven groups. NS, dexamethasone (1.0 μmol/L) and As2O3 at concentrations of 0.25, 0.5, 1.0, 2.0, 4.0 μmol/L were instilled respectively into cultured FB. The effects of different concentration of As2O3 and dexamethasone on cell growth were observed at 1, 3, 5 and 7 day. The changes of cell cycle and the positive expression rate of c-myc and c-sis were examined by flow cytometry (FCM). RESULTS: Various concentrations of As2O3 significantly inhibited the proliferation of FB in vitro and showed a dose-dependent and time-dependent tendency. Data from FCM indicated that G1-phase cell percentage increased and G2/M-phase cell percentage decreased with the increase in As2O3 concentrations. The expression of c-myc and c-sis was significantly inhibited by As2O3 (2.0, 4.0 μmol/L). CONCLUSION: As2O3 inhibits FB proliferation by downregulating the expression of c-myc and c-sis.