结核分枝杆菌Rv3407蛋白抗原表位的预测

目的预测结核分枝杆菌Rv3407的抗原表位。方法利用DNAStar软件包中Protean软件对Rv3407氨基酸序列进行分析,采用包括二级结构、亲水性、抗原性、表面可能性、柔韧性等多参数预测其二级结构及T细胞和B细胞抗原表位。结果 Rv3407蛋白具有丰富的二级结构和多处抗原指数较高的区段,潜在的B细胞抗原表位较少,可能位于11-26、28-43、50-61、66-74、76-99位氨基酸残基或其附近,这些区域基本上含有β转角结构,亲水性、表面可能性和柔韧性指数都较高。该蛋白潜在的T细胞抗原表位较多,可能位于2-12、22-26、34-37、40-44、56-60、75-79、86-93位氨基酸残基或其附近。结论结核分枝杆菌Rv3407是一个T细胞抗原表位占优势的蛋白抗原,B细胞抗原表位略少,预测结果为该蛋白抗原表位的进一步研究与应用奠定了基础。

Prediction of epitopes of Mycobacterium tuberculosis Rv3407 protein

Objective To predict the epitopes of Mycobacteriurn tuberculosis Rv3407 protein. Methods The amino acid sequence of Rv3407 protein was analyzed using Protean in DNAStar and B cell and T cell epitopes were predicted using multiple parameters, including the secondary structure, hydrophilicity, antigenicity, surface probability, and flexi-bility. Results The Rv3407 protein has an extensive secondary structure and multiple regions with high antigenicity. There were a few potential B cell epitopes at or near amino acids 11--26, 28--43, 50--61, 66--74, and 76--99. Theses epitopes had greater antigenicity, a beta turn, and a high level of hydrophilicity, surface probability, and flexibility. There were potential T cell epitopes at or near amino acids 2--12, 22--26, 34--37, 40--44, 56--60, 75--79, and 86-- 93. Conclusion M. tuberculosis Rv3407 protein is an antigen with a dominant T-cell epitope, B cell epitopes also exist but are less prevalent than T cell epitopes. This prediction of epitopes has laid the foundation for the further study and use of this protein.