促进体外培养人鼻中隔软骨细胞增殖方法的研究

目的 :研究促进人鼻中隔软骨细胞在体外增殖的方法 ,为人鼻中隔软骨细胞在软骨组织工程的应用提供方法和理论依据。方法 :研究三种培养基 (DMEM、F- 12、16 4 0 )、胰岛素、琼脂和肌苷对正常成人鼻中隔软骨细胞体外培养的影响。结果 :16 4 0培养基和新生牛血清适合进行人鼻中隔软骨细胞培养 ,胰岛素可保持软骨细胞生长 ,琼脂可促进软骨细胞表型稳定 ;在 16 4 0培养基条件下 ,肌苷对软骨细胞的生长无明显的促进作用。

A study of accelerating proliferation of human nasal septal chondrocytes cultured in vitro

Objective:To study the proliferation method of human nasal ceptal chondrocytes culture in vitro.Tissue engineering offered a new way to cartilage reconstruction.To select and culture proper seeds of chondrocytes was the basis of cartilage tissue engineering .Septal cartilage was easy to obtain,it was possible to use septal chondrocytes in tissue engineering study and finally in clinical application.Methods:The effect of DMEM,F 12,1640 culture medium and insulin,agar and inosin on normal human nasal septal chondrocytes cultured in vitro were compared.Results:It showed that the proliferation of human nasal septal chondrocytes was better in the 1640 culture medium with insulin than other mediums. [Chinese Arch Otolaryngol Head Neck Surg,2000;7(5):294~297 From the Department of Otolaryngology,Zhujiang Hospital,First Military Medical University,Guangzhou 510282 (Dr.Huang Jinzhong)]