新生儿溶血病检测试验多样性与胆红素水平相关性研究

目的探讨新生儿ABO溶血病（ABO．HDN）溶血三项试验不同结果与胆红素水平的相关性。方法对85例ABO—HDN患儿进行血清溶血三项试验[抗人球蛋白试验（DAT）、游离抗体试验、抗体释放试验]、母亲IgG抗A（B）抗体、血清胆红素等测定。结果ABO．HDN患儿DAT阳性者血清总胆红素峰值与DAT阴性者比较，差异有统计学意义（P〈0．05，0．01）；DAT阴性间比较、DAT阳性问比较（P〉0．05），差异无统计学意义。新生儿ABO．HDN患儿母亲血型抗体与血清总胆红素水平比较，差异无统计学意义（P〉0．05）。结论ABO-HDN患儿DAT阳性合并抗体释放试验阳性或三项试验均阳性时，血清胆红素水平增高较明显，DAT阴性释放试验和／或游离试验阳性时，新生儿ABO-HDN相对较轻，而母亲IgG抗体效价高低不能反映HDN的严重程度，这可为临床合理治疗HDN提供帮助。

Research on the relevance between different results of hemolysis detection tests and serum bilirubin levels of hemolytic disease of the newborn

Objective To study the relevance between different results of three hemolysis detection tests and serum bilirubin levels of ABO hemolytic disease of the newborn(ABO-HDN).Methods Serological hemolysis three tests (direct anti-human globulin test,free antibody test,antibody release test),maternal anti-A (B)IgG antibodies,serum bilirubin were examined in 85 cases of ABO-HDN.Results There were significant differences in levels of total serum bilirubin (TSB) between groups of DAT-positive and groups of DAT-negative (P ＜ 0.05,or P ＜ 0.01).There weren' t significants difference between groups of DAT-positive or groups of DAT-negative in levels of total serum bilirubin(P ＞ 0.05).There wasn' t significant difference between maternal antibodies titer and levels of total serum bilirubin (P ＞ 0.05).Conclusion When DAT is positive,the serum bilirubin level was significantly increased,and maternal antibodies titer couldn' t show severity of ABO-HDN.It could be helpfull for the clinical treatment of ABO-HDN.