长春花叶片愈伤组织诱导及培养过程中生物碱合成类型的变化和调节及有关酶的分析

在４种不同培养基上诱导长春花叶片愈伤组织的过过程中，叶片中含量较高的文多灵和长春质碱迅速降解有低水平，而叶片中含量较低的阿玛码和蛇根碱的合成却逐渐增加，同时酸性和碱性过氧化物酶活性呈上升趋势，尤其碱性过氧化物酶与蛇根碱和阿玛碱的相关性较密切。

Regulation of Indole Alkaloids Synthesis and Related Enzymes During Callus Initiation and Culture of Madagascar Periwinkle Leaves (Catharanthus roseus)

Leaf calluses of Catharanthus roseus (L.) G.Don were initiated in four different media under illumination or in dark. Visible callus began to form within 10 to 15 days. The ordinarily high contents of vindoline and catharanthine in the leaf declined rapidly, while the lower ajmalicine and serpentine contents increased markedly during callus initiation. At the same time, acidic and alkaline peroxidase showed a tendency to increase their activities, especially the activity of alkaline peroxidase was more closely related with the synthesis of serpentine and ajmalicine. Results also showed that illumination improved occurrence of leaf callus in advance and stimulated peroxidase activity, beneficial for the synthesis of vindoline, catharanthine and serpentine. 2.4-D represses biosynthesis of indole alkaloids and peroxidase activity in all cultures.