| HPLC法测定红毛五加皮中绿原酸的含量 |
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| 引用本文： | 钟世红,卫莹芳,古锐.HPLC法测定红毛五加皮中绿原酸的含量[J].四川中医,2010(3):40-41. |
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| 作者姓名： | 钟世红 卫莹芳 古锐 |
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| 作者单位： | 成都医学院药学院;成都中医药大学教育部中药材标准化重点实验室;成都中医药大学; |
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| 摘 要： | 目的:建立红毛五加皮中绿原酸的含量测定方法,并测定不同产地样品。方法:采用乙睛-0.3%磷酸二氢钠水溶液梯度洗脱系统;流速:1.0ml/min;柱温:35℃;检测波长为324nm。结果:绿原酸的线性范围为0.270～2.163μg,平均加样回收率为102.13%,RSD小于2%。结论:该方法简便、准确、重现性好,可为红毛五加皮的质量控制提供参考。
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| 关 键 词： | 红毛五加皮 绿原酸 HPLC 高效液相色谱 |
HPLC Determination of Chlorogenic Acid in Cortex Acanthopanacis Giraldii |
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| Zhong Shihong Wei Yingfang Gu Rui .Pharmaceutical School,Chengdu Medical College.HPLC Determination of Chlorogenic Acid in Cortex Acanthopanacis Giraldii[J].Journal of Sichuan of Traditional Medicine,2010(3):40-41. |
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| Authors: | Zhong Shihong Wei Yingfang Gu Rui Pharmaceutical School Chengdu Medical College |
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| Institution: | Zhong Shihong1 Wei Yingfang2 Gu Rui3 1.Pharmaceutical School,Chengdu Medical College(Chengdu Sichuan 610083 China) 2.The Ministry of Education key Laboratory of St,ardization of Chinese herbal medicines in Chengdu University of TCM(Chengdu Sichuan 611131,China) 3.Chengdu University of TCM(Chengdu Sichuan 611131,China) |
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| Abstract: | Objectives： To determine chlorogenic acid in Cortex acanthopauacis Giraldii. And determine samples from different regions. Methods ： A RP-HPLC method was used for the determination. A mobile phase program of gradient elution with acetonitrile and 0. 3 % sodium dihydrogen phosphate at a flow rate of 1. 0 ml/min was employed. The column temperature was 35℃. The detection wavelength was 324nm. Results： The linear range of chlorogenic acid was 0. 270 -2. 163ug. The average recovery was 102. 13% and the RSD was under 2%. Conclusions： The method is simple, accurate and with a good reproducibility. It is useful for the quality control of Cortex aeanthopanacis Giraldii. |
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| Keywords: | Cortex Acanthopanacis Giraldii chlorogenic acid HPLC fingerprint |
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