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基于生物学原理的农药残留检测技术研究进展
引用本文:何永红,高志贤,晁福寰.基于生物学原理的农药残留检测技术研究进展[J].卫生研究,2004,33(1):112-114.
作者姓名:何永红  高志贤  晁福寰
作者单位:军事医学科学院卫生学环境医学研究所,天津,300050
基金项目:国家 8 63计划青年项目 (No .2 0 0 2AA6491 80 ),国家科技攻关计划资助项目 (No .2 0 0 1BA80 4A1 4 )
摘    要:综述了近年来在农药残留分析中应用的一些基于生物学原理的检测技术 ,包括免疫分析法、生物传感器、酶分析技术和活生物体分析技术等。免疫分析法特异、灵敏 ,但农药抗体的制备并非易事 ;生物传感器具有实时、快速的优点 ,但灵敏度和稳定性仍需提高 ;酶分析技术和活生物体分析技术操作简单、成本低 ,可用于急性中毒定性和污染严重的检测。上述技术的共同特点是 ,一般不需大型设备、检测快速、成本低 ,因此 ,在大规模农药残留监测和实时现场检测方面具有广泛的应用前景。

关 键 词:农药残留  生物技术
文章编号:1000-8020(2004)01-0112-03
修稿时间:2003年4月27日

Advance of pesticide residue analysis techniques based on biological principle
He Yonghong,Gao Zhixian,Chao Fuhuan Institute of Hygiene and Environment Medicine,Academy of Military Medical Sciences,Tianjin ,China.Advance of pesticide residue analysis techniques based on biological principle[J].Journal of Hygiene Research,2004,33(1):112-114.
Authors:He Yonghong  Gao Zhixian  Chao Fuhuan Institute of Hygiene and Environment Medicine  Academy of Military Medical Sciences  Tianjin  China
Institution:Institute of Hygiene and Environment Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Abstract:The application of pesticide residue analysis techniques based on biological principle, such as immunoassay, biosensor and direct test the influence of pesticide upon the activity of the enzyme and the growth of the propagation, was reviewed in this paper. Immunoassay is a kind of precise and sensitive method, but it's not easy to produce the antibody of pesticide. The strongpoint of biosensor is the process of the detection is quickly and real time, but the sensitivity and stability of biosensor still need improve. The tests based on the activity of the enzyme and the growth of the propagation are simple and inexpensive, and are useful in the situation of acute-poisoning and severe contamination. These techniques general work fast, cost inexpensive, and do not need large-volume unit, so they have a good large potential in extensive pesticide residue monitoring and spot assays.
Keywords:pesticide residues analysis  biotechnique
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