Treg细胞在骨髓增生异常综合征中的变化及其意义

目的 研究CD4+Treg细胞在骨髓增生异常综合征(MDS)中的变化及其意义.并探讨CD4+Treg细胞在MDS低危组和MDS高危组中的变化和内在联系.方法 流式细胞仪检测MDS低危组(21例)、MDS高危组(15例)及正常对照组(22例)CD4+CD25+T细胞和CD4+FOXP3+T细胞分别占CD4+T细胞的比例,采用直接免疫荧光法标记T细胞特征抗原.结论 CD4+FOXP3+T细胞占CD4+T细胞的比例在各组中比较:MDS低危组与高危组均明显高于正常对照组,MDS高危组高于低危组,差异有统计学意义,CD4+CD25+T细胞占CD4+T的比例在各组中无明显差异.结论CD4+Treg细胞在MDS组较正常对照组明显升高,并且随着疾病的进展CD4+Treg细胞逐渐升高.

Alterations and significance of Treg cells in myelodysplastic syndromes

Objective To explore the alterations and significance of regulatory T (Treg) cells in patients with myelodysplastic syndromes (MDS) and to investigate the distinction and association of Treg cells a-mong MDS low-risk group and MDS high-risk group. Methods The proportion of CD4~+ CD25~+ T cells and of CD4 ~+ FOXP3~+ T cells in CD4~+ T cells were examined by direct immunofluorescence straining technique and detected by flow cytometry. There were three groups in the study which were MDS low-risk group (21 cases), MDS high-risk group (15 cases), normal control group (22 cases). Results The proportion of CD4 ~+ FOXP3~+ T cells in patients with MDS (both high-risk group and low-risk group ) was also significantly higher than normal control group (P <0.05). The percentages of CD4~+ FOXP3 ~+ T cells were distinct statistically between low-risk group(7.46 ±2.01)% and high-risk group(9.62±3.61)% , and the later was higher than the former (P <0. 05). But there was no significant difference in the percentages of CD4~+ CD25~+ T cells in the peripheral blood between any groups (P > 0. 05). Conclusion The proportion of Treg cells is much higher in MDS patients than in normal controls and gradually increased while the disease progressed.