极低频电磁辐射下HFSF中MMP-2的表达及作用

目的研究极低频电磁场（ELF-EMF）作用下人胚胎眼巩膜成纤维细胞（HFSF）中基质金属蛋白酶-2（MMP-2）活性与蛋白表达的变化以及ELF-EMF对I型胶原（Collagen I）合成的影响。方法实验研究。根据是否暴露于50 Hz、0.2 mT电磁场或加入MMP-2特异性抑制剂（sc-204092），将实验细胞分为对照组、辐照组和抑制剂组。明胶酶谱法检测各组HFSF中MMP-2酶活性的变化；Western Blot法检测各组HFSF中MMP-2、Collagen I蛋白水平的变化。采用单因素方差分析进行数据分析。结果3组HFSF细胞中MMP-2酶活性、MMP-2、Collagen I蛋白表达比较差异均具有统计学意义（F=14.96、139.88、63.46，P均<0.01）；辐照组较对照组MMP-2酶活性和蛋白表达升高（P<0.05），Collagen I蛋白表达下降（P<0.01）；抑制剂组较辐照组MMP-2酶活性和蛋白表达下降（P<0.01），Collagen I蛋白表达升高（P<0.05）。结论ELF-EMF可能通过影响体外培养的HFSF中MMP-2的酶活性和蛋白表达来调节Collagen I的合成。

Expression and effect of matrix metalloproteinase 2 in human fetal scleral fibroblasts treated with an extremely low frequency electromagnetic field

ObjectiveTo observe the matrix metalloproteinase 2 （MMP-2） activity and protein expression in human fetal scleral fibroblasts （HFSFs） exposed to an extremely low frequency electromagnetic field （ELF-EMF） and its impact on collagen I synthesis. MethodsThis was an experimental study． HFSFs were cultured and divided into a control group， radiation group and inhibitor group according to their exposure to 50 Hz， 0.2 mT electromagnetic field or joint MMP-2 specific inhibitors （sc-204092）. MMP-2 activity was measured by gelatin enzymography. MMP-2 and collagen I protein expression were detected by Western Blot． A single factor analysis of variance was used to determine whether there was a difference among these groups. ResultsThere were significant differences between the three groups （F=14.96， 139.88， 63.46， P<0.01）. Compared to the control group， MMP-2 activity and protein expression increased in the radiation group （P<0.05） and collagen I protein expression decreased （P<0.01）. Compared to the radiation group， MMP-2 activity and protein expression decreased in the inhibitor group （P<0.01） and collagen I protein expression increased （P<0.05）. ConclusionELF-EMF may regulate the synthesis of collagen I by affecting the activity and protein expression of MMP-2.