视频跟踪-算机自动分析的小鼠条件性位置偏爱实验系统的构建

目的：建立视频跟踪-计算机自动分析的小鼠条件性位置偏爱实验系统。方法：位置偏爱系统主要由电脑、摄像头、活动盒、隔音箱以及相应的分析软件组成。用吗啡诱导的小鼠条件性位置偏爱实验评价建立的位置偏爱实验系统，同时研究条件化训练过程中，吗啡对小鼠在伴药盒的运动活性的影响。结果：同生理盐水对照组相比，腹腔注射低(1mg/kg)、中(3mg/kg，5mg/kg)、高(10mg/kg)剂量的吗啡都能显著延长小鼠在伴药盒的停留时间，但无明显剂量反应关系。并且中、高剂量的吗啡显著增强小鼠在伴药盒内运动活性，其中5mg/kg、10mg/kg吗啡诱导运动敏感化形成。结论：成功建立视频跟踪-计算机自动分析的小鼠条件性位置偏爱实验系统，该系统可靠、稳定。

Establishment of computer-based video-tracking conditioned place preference experiment system in mice

OBJECTIVE: To establish the computer-based video-tracking conditioned place preference (CPP) system in mice. METHODS: The CPP system was composed of computer, camera, soundproof box, shuttle box and analytical software. The results of morphine-induced conditioned place preference were used to evaluate the experiment system. And the effect of morphine-induced locomotor activity in drug-paired compartment was studied in mice. RESULTS: Low (1 mg/kg, i.p.), moderate (3 mg/kg, 5 mg/kg, i.p.) and high (10 mg/kg, i.p.) dose of morphine significantly prolonged the time mice spent in drug-paired compartment compared with saline, but there was no dose-response relation. Moderate and high dose of morphine significantly enhanced locomotor activity, among which 5 mg/kg and 10 mg/kg morphine induced behavior sensitization in drug-paired compartment during the conditioning sessions. CONCLUSION: The computer-based video-tracking conditioned place preference experiment system in mice established successfully is reliable and stable.