| 高效液相色谱法测定乐脉片中芍药苷含量 |
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| 引用本文： | 封海霞,况刚,王慧,徐小利,袁祥慧,邓开英. 高效液相色谱法测定乐脉片中芍药苷含量[J]. 中国药业, 2013, 0(22): 54-55 |
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| 作者姓名： | 封海霞 况刚 王慧 徐小利 袁祥慧 邓开英 |
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| 作者单位： | 重庆市食品药品检验所,重庆401121 |
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| 摘 要： | 目的建立测定乐脉片中芍药苷含量的高效液相色谱法。方法色谱柱为Waters Symmetry Shield RPl8柱（150mm×3．9mm，5μm），流动相为乙腈-0．05％磷酸（13：87），检测波长230nm。结果芍药苷进样量在0．20403—4．08060μg范围内与峰面积呈良好的线性关系，r=0．9995。平均回收率为100．52％，RSD=2．03％（n=6）。结论该方法简便、快速准确、灵敏度高、重现性好，可作为乐脉片中芍药苷的含量测定法。
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| 关 键 词： | 乐脉片 芍药苷 高效液相色谱法 含量测定 |
Determination of Paeoniflorin in Lemai Tablets by HPLC |
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| Feng Haixia,Kuang Gang,Wang Hui,Xu Xiaoli,Yuan Xianghui,Deng Kaiying. Determination of Paeoniflorin in Lemai Tablets by HPLC[J]. China Pharmaceuticals, 2013, 0(22): 54-55 |
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| Authors: | Feng Haixia Kuang Gang Wang Hui Xu Xiaoli Yuan Xianghui Deng Kaiying |
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| Affiliation: | (Chongqing Municipal Institute for Food and Drug Control, Chongqing, China 401121) |
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| Abstract: | Objective To establish a method for the determination of the paeonifiorin content in Lemai Tablet by HPLC. Methods The HPLC system consisted of the Waters SymmetryShield RP18 column（150 mm x3.9 mm,5 μm） with acetonitrile- 0.05% phos- phoric acid（ 13：87） as the mobile phase,the detection wavelength was set at 230 nm. Results The sample size of paeoniflorin in the range of 0.204 03-4.080 60 μg had the good linear relation with the peak area. The average recovery rate was 100.52% （n=6, RSD = 2.03% ）. Conclusion This method is convenient, quick and accurate with hith sensitivity an dgood reproducibility,which is suit- able for the determination of the paeoniflorin content in Lemai Tablet. |
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| Keywords: | Lemai Tablets paeoniflorin HPLC content determination |
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