当归中一种杂多糖的分离纯化与结构鉴定

 目的研究从当归[Angelica sinensis(Oliv)Diels]中分离纯化获得的均一多糖ASDII-3-3的结构。方法采用糖组成分析、甲基化分析以及NMR确定该多糖的结构。结果该均一多糖相对分子质量为44000,主要由阿拉伯糖和半乳糖组成,此外尚含有少量的鼠李糖、甘露糖和木糖;其中阿拉伯糖的连接方式为端基和1,5连接;半乳糖的连接方式为1,4和1,4,6连接;鼠李糖为1,2连接,甘露糖为端基,木糖为1,4连接。结论该均一多糖为首次从当归中分离获得的一种新的杂多糖。

Isolation and Structure Analysis of a Hetero-Polysaccharide from Angelica sinensis (Oliv)Diels.

OBJECTIVE To characterize the chemical structure of a homogenous polysaccharide ASDII-3-3 purified from Angelica sinensis(Oliv)Diels.METHODS Sugar composition analysis,methylation analysis and NMR were used to determine the structural features.RESULTS The molecular weight of the homogenous polysaccharide was 44 000 dalton estimated by HPGPC,mainly composed of arabinose and galactose,as well as xylose,rhamnose and mannose. The linkage models of arabinose were terminal and 1,5 linked,galactose contained 1,4 and 1,4,6 linked units,xylose was 1,4 linked,rhamnose was 1,2 linked and mannose was terminal.CONCLUSION This polysaccharide is a new hetero-polysaccharide for the first time from Angelica sinensis(Oliv)Diels.