汉防己碱和MK801对小鼠急性一氧化碳中毒脑损伤的保护作用

目的 研究汉防己碱和MK801对小鼠急性一氧化碳(CO)中毒致脑损伤是否有保护作用。方法 小鼠腹腔注射CO150ml／kg，间隔3h注射1次，连续3次，在每次给予CO前30min腹腔注射汉防己碱15mg／kg和MK8012mg／kg；以CO中毒后小鼠被动回避性学习记忆能力改变、脑组织病理学和Ca^2 Mg^2 -ATPase活力改变为指标，观察汉防己碱和MK801对CO中毒致小鼠迟发性脑损伤的保护作用。结果 给予汉防己碱能明显改善小鼠因CO中毒引起的学习记忆巩固能力障碍，防止海马神经元细胞病理性损伤，阻遏CO中毒引起的小鼠脑组织Ca^2 Mg^2 -ATPase活力的降低；而MK801对CO中毒后小鼠被动回避性学习记忆能力及脑组织病理学和Ca^2 Mg^2 -ATPase活力改变均无明显影响。结论 汉防己碱对急性CO中毒致小鼠脑损伤有明显的保护作用，而MK801至少在本次研究剂量下无明显的保护作用。

Protective Effects of Tetrandrine and MK801 on Cerebral Injury Induced by Acute Carbon Monoxide Intoxication in Mice

Objective To study the effects of tetrandrine and MK801 on cerebral injury in mice due to acute carbon monoxide intoxication.Methods Mice were exposed to CO( 150 ml/kg ip) once every 3h, successively for 3 times.Either tetrandrine (15 mg/kg) or MK801(2 mg/kg) was administrated 30 min before CO exposure.Following items were investigated:changes of memory in passive avoidance test,the pathomorphological observation of brain tissue slices and changes of Ca 2+ Mg 2+ ATPase activities in cerebral tissue.Results Results showed that pre administration with tetrandrine could significantly conserve the impairment of memory function, prevent the cell death in hippocampal neurons and hinder the descending of Ca 2+ Mg 2+ ATPase activity induced by acute CO poisoning.However,similar effects were not observed with MK801.Conclusions These results suggest that pretreatment of tetrandrine could markedly prevent mice from cerebral injury after acute CO poisoning.