中药五加皮提取成分抗肿瘤活性作用机理研究

目的 研究五加皮提取成分（Acanthopanax gracilistylus extract，Age）对肿瘤细胞的作用机理。方法 采用3^H]-TdR掺入法测定肿瘤细胞增殖反应；用流式细胞技术分析Age对肿瘤细胞周期的影响；用蛋白印迹技术检测Age对肿瘤细胞Rb（retinoblastoma）、Cdk（cyclin-dependent kinases）等酶类的影响。结果 体外细胞活力试验证明，Age仅抑制肿瘤细胞增殖，并不导致细胞死亡。在Age的作用下，肿瘤细胞增殖停止在细胞周期的G0／G1期，而未见直接的细胞毒效应。Age可诱导Rb、Cdk2和Cdk4表达降低，使细胞停止增殖。结论 Age对肿瘤细胞的作用机制是通过调节控制细胞周期的酶类而发挥作用的。

Study on Mechanism of the Anti-tumor Activity of Acanthopanax Gracilistylus

OBJECTIVE: To study the mechanism of anti-tumor activity of Acanthopanax gracilistylus extract (Age). METHODS: The tumor cells proliferation was detected by using (3H)-TdR incorporation method, and the effects of Age on cell cycle of tumor cells, retinoblastoma (Rb) protein and cyclin-dependent kinases (Cdk) were analyzed by flow cytometry and Western blotting assay, respectively. RESULTS: It was indicated by cytoactivity test in vitro that Age only had effect in inhibiting the proliferation of tumor cells, it couldn't lead to death of cells. Under action of Age, the proliferation of tumor cells was halted at G0/G1 stage of cell cycle, and showed no direct cytotoxic effect by Age. Age could induce lowering of the expression of Rb, Cdk2 and Cdk4, cause halt of tumor cell proliferation. CONCLUSION: The tumor inhibitory effect of Age is realized by way of regulating the activity of cell cycle controlling enzymes to suspend the proliferation of tumor cells.