In vitro culture of human bone marrow mesenchymal stem cell clonies and induced differentiation into neuron-like cells. |
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Authors: | Jing-ren Lin Kun-yuan Guo Jiang-qi Li Ding-an Yan |
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Institution: | Department of Hematology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. |
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Abstract: | OBJECTIVE: To study the long-term in vitro culture of human bone marrow mesenchymal stem cells (hMSC) and their phenotypical and functional properties. METHODS: Adherent hMSC colonies were digested by 0.25% trypsin-EDTA with a clone cycle for in vitro subculture. Flow cytometry was employed to examine the phenotypes of the cells. Their committed differentiation potential to neurons, clone-forming ability and growth curves were all investigated. RESULTS: hMSCs could be subcultured under this culture condition for 20 passages, expressing CD13, CD29 and CD59 but not CD11, CD14, CD31, CD34, CD45, CD80, CD86, CD117 and HLA-DR. The cells could be induced to differentiate into neurons when subcultured for 17 passages. CONCLUSION: hMSCs can be efficiently expanded under this culture condition, and the colony-derived hMSCs can maintain the differentiation potentials and retain their biological characteristics. |
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