癌旁组织中FasL表达与淋巴细胞凋亡的关系

目的通过对人体肿瘤标本的连续切片,测定同一区域肿瘤周边病灶中淋巴细胞上的FasL和肿瘤浸润淋巴细胞凋亡的关系,探讨肿瘤免疫逃避的机制.方法应用免疫组织化学法染色检测30例肿瘤病理标本中FasL的表达和淋巴细胞凋亡,采用显微图像分析系统进行平均灰度值测定.结果肿瘤周边病灶中淋巴细胞上的FasL和淋巴细胞凋亡的平均灰度值之间具有相关性(r＝0.757),且有统计学意义(P<0.001).结论肿瘤细胞能通过主动分泌FasL,并与肿瘤浸润淋巴细胞(TIL)上的Fas受体结合,进而诱导淋巴细胞凋亡,从而成功逃避机体的免疫攻击.

Relationship between the expression of FasL and apoptosis of TIL in paracancer tissues

Objective To determine whether human adenocarcinomas FasL expression is associated with increased apoptotic induction in tumor-infiltrating lymphocyte (TIL) and to provide evidence to support the Fas counterattack theory as a mechanism of immune escape in cancer.Methods Expression of FasL and apoptosis in 30 patients with cancer were determined by immunohistochemical and TUNEL method with serial sections in same area of the tumor.The average grey value was evaluated by microscope image analyzing system.Results The average value of FasL and apoptosis on TIL in cancer district was detected positive in 30 patients with cancer. Special correlation existed between the average value of FasL and apoptosis on TIL(r=0.757,P<0.001).Conclusion FasL-expressing regions of pericancer tissue were demonstrated.This finding suggests Fas-mediated apoptotic depletion of TIL in response to FasL expression in cancers, and provides evidence to support the Fas counterattack theory as a mechanism of immune escape in cancers.