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| RP-HPLC法测定麻黄配方颗粒中麻黄碱和伪麻黄碱的含量 | |
| 引用本文： | 周斌,刘可越,刘海军,程春清,张铁军.RP-HPLC法测定麻黄配方颗粒中麻黄碱和伪麻黄碱的含量[J].天津药学,2009,21(4):9-11. |
| 作者姓名： | 周斌 刘可越 刘海军 程春清 张铁军 |
| 作者单位： | 1. 江西科技师范学院药学院,南昌,330013 2. 九江学院医学院,九江,332000 3. 天津药物研究院,天津,300193 |
| 基金项目： | 江西省教育厅资助项目 |
| 摘 要： | 目的：建立用反相高效液相色谱法测定麻黄配方颗粒中麻黄碱和伪麻黄碱含量。方法：采用BonChrom—C18柱。流动相为乙腈-0．02mol/L磷酸二氢钾-磷酸（5：95：0．1），柱温为35℃，检测波长为207nm，流速为1．0ml／min。结果：盐酸麻黄碱线性范围0．03305-0．3966μg，r=0．9999，平均回收率为100．42％，RSD为1．78％（n=6）；盐酸伪麻黄碱的线性范围为0．023～0．276μg，r=0．9997，平均回收率为99．27％，RSD为2．05％（n=6）。结论：本法重复性好，可作为麻黄配方颗粒中麻黄碱和伪麻黄碱的定量分析方法。 |
| 关 键 词： | HPLC 麻黄碱 伪麻黄碱 配方颗粒 麻黄 |
Determination of ephedrine hydrochioride and pseudo ephedrine hydrochloride in Ephedra Dispensing Granules by RP -HPLC |
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| Zhou Bin,Liu Keyue,Liu Haijun,Cheng Chunqing,Zhang Tiejun.Determination of ephedrine hydrochioride and pseudo ephedrine hydrochloride in Ephedra Dispensing Granules by RP -HPLC[J].Tianjin Pharmacy,2009,21(4):9-11. | |
| Authors: | Zhou Bin Liu Keyue Liu Haijun Cheng Chunqing Zhang Tiejun |
| Institution: | Zhou Bin , Liu Keyue, Liu Haijun , Cheng Chunqing , Zhang Tiejun ( 1. School of Pharmacy, Jiangxi Sciencie and Technology Normal University, Nanchang 330013 ; 2. Medical College,Jiujiang Uni- versity ,Jiujiang 332000 ;3. Department of Modernization of Chinese Traditional Medicine, Tianjin Institute of Pharmaceutical Research, Tianjin 300193) |
| Abstract: | Objective ： To set up the method for determination of ephedrine hydrochloride and pseudo ephedrine hydrochloride in Ephedra sinica Dispensing Granules by RP - HPLC. Methods： The BonChrom - C18 column（4.6 mm × 200 mm,5μm）maintained at 35 ℃ with acetonitrile - 0.02 mol/L KH2 PO4 - H3 PO4 （5 ： 95： 0.1 ） as the mobile phase was used . The flow rate was at 1.0 ml/min and the detective wavelength was at 207 nm. Results ： The calibration curve was linear in the range of 0.033 05 to 0.396 6μg for ephedrine hydrochloride （ r = 0.999 9） ; the average recovery was 100.42 % and RSD was 1.78 % （n = 6）. The calibration curve was linear in the range of 0. 023 to 0. 276 μg for pseudo ephedrine hydrochloride （ r = 0.999 7 ）, the average recovery was 99.27 % and RSD was 2.05 % （ n = 6）. Conclusion ： The method was available with a good reproducibility and can control the quality of Ephedra sinica Dispensing Granules. |
| Keywords: | HPLC |
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